1996年
The major component of Na-CBZ-L-Lysine thiobenzyl ester (BLT)-specific proteases in cytoplasmic granules of murine intraepithelial lymphocytes is granzyme A
Immunobiology
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- 巻
- 196
- 号
- 5
- 開始ページ
- 465
- 終了ページ
- 474
- 記述言語
- 英語
- 掲載種別
- 出版者・発行元
- Elsevier GmbH
Na-CBZ-L-lysine thiobenzyl ester (BLT)-specific proteases in cytoplasmic granules of intraepithelial lymphocytes in the murine intestine (iIEL) were characterized. BLT-specific proteases were isolated with the Sephacryl S-200 column chromatography, and the sample isolated contained a protein with a molecular weight of 58 kDa. The 58 kDa protein consisted of the homodimer of the 30 kDa subunits. The 58 kDa protease was detected by [<
sup>
3<
/sup>
] diisopropylfluorophosphate (DFP)-labeling, and also detectable by the immunoblotting using an antibody against the partial synthetic peptide of granzymce A. The cytoplasmic granules of iIEL were stained positively by an immunofluorescence with anti-granzyme A antibody. Therefore, it was suggested that the major BLT-specific proteases present in cytoplasmic granules of iIEL, might be granzyme A.
sup>
3<
/sup>
] diisopropylfluorophosphate (DFP)-labeling, and also detectable by the immunoblotting using an antibody against the partial synthetic peptide of granzymce A. The cytoplasmic granules of iIEL were stained positively by an immunofluorescence with anti-granzyme A antibody. Therefore, it was suggested that the major BLT-specific proteases present in cytoplasmic granules of iIEL, might be granzyme A.
- リンク情報
- ID情報
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- ISSN : 0171-2985
- PubMed ID : 9145324
- SCOPUS ID : 0030424059