MISC

2000年3月

Papaverine combined with prostaglandin ea synergistically induces neuron-like morphological changes and decrease of malignancy in human prostatic cancer LNCaP cells

ANTICANCER RESEARCH
  • T Shimizu
  • ,
  • Y Ohta
  • ,
  • H Ozawa
  • ,
  • H Matsushima
  • ,
  • K Takeda

20
2A
開始ページ
761
終了ページ
767
記述言語
英語
掲載種別
出版者・発行元
INT INST ANTICANCER RESEARCH

We are interested in the possibility of new prostate cancer therapy that would control tumor malignancy via the induction of terminal cell differentiation Here, we investigated the combined effect of various cAMP reagents an LNCaP human prostate carcinoma cells. Papaverine and prostaglandin E-2 (PGE(2)), combined synergistically induced morphological changes. Electron microscope study suggested that cells treated with both reagents become like neuroendocrine cells. We then investigated the effect of both reagents on proliferation and malignancy of LNCaP cells. The malignancy of cells was analyzed by soft agar colony-forming assay and an in vitro invasion assay Proliferation and malignancy of LNCaP cells treated with both reagents were significantly decreased in comparison to the proliferation and malignancy of untreated cells. Furthermore, the expression of oncogenes such as c-myc and Bcl-2 was suppressed in differentiated LNCaP cells. These results suggest that papaverine combined with PGE2 can synergistically induce neuronal differentiation as well as decrease the malignancy of human prostatic cancer LNCaP cells.

Web of Science ® 被引用回数 : 12

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000086764700017&DestApp=WOS_CPL

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