論文

査読有り
2009年6月

Synergistic induction of gene expression during the differentiation into mature macrophage in human myeloblastic leukemia cells treated with TPA and KH1060

LEUKEMIA RESEARCH
  • Takahisa Shimizu
  • ,
  • Aya Kuromi
  • ,
  • Ken Takeda

33
6
開始ページ
803
終了ページ
809
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.leukres.2008.11.020
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

Treatment of human myeloblastic leukemia ML-1 cells with the phorbol ester TPA in combination with the vitamin D(3) analogue KH1060 will induce a synergistic differentiation to Mature macrophage with multinuclei. To investigate the mechanism Underlying this differentiation and the synergistic effect, a cDNA microarray and Northern blot analysis were used to examine gene expression profiles of ML-1 treated with TPA and/or KH1060. Results show that KH1060 enhanced several TPA-induced gene cells expressions and that TPA enhanced several KH1060-induced gene expressions. Studies with inhibitors of signaling molecules suggested that PKC and MAPK pathways play an important role in the differentiation induced by TPA and KH1060, and that they are associated with the synergistic induction of genes. The results of this study indicate the possibility that the expression of various genes are induced synergistically by cross-talk between TPA and KH1060 signals. It is likely that the synergistic effect on gene expression leads to the synergistic induction of differentiation by both reagents. (C) 2008 Elsevier Ltd. All rights reserved.

Web of Science ® 被引用回数 : 2

リンク情報
DOI
https://doi.org/10.1016/j.leukres.2008.11.020
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000265771600017&DestApp=WOS_CPL

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