© 2017 IEEE. Contact manipulation using a micropipette is widely used for cell culture and evaluation. However, the operation efficiency and the damage on the cells depend on the individual skills. A technique for manipulating a micro biological material with high accuracy and selectivity is required. Our group has been investigating noncontact ultrasonic manipulation techniques using acoustic standing wave. In this report, ultrasonic manipulation, separation, and evaluation of HeLa cells in a microchannel are discussed.