2020年
Biological Properties of the Aggregated Form of Chitosan Magnetic Nanoparticle
In Vivo
- 巻
- 34
- 号
- 4
- 開始ページ
- 1729
- 終了ページ
- 1738
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.21873/invivo.11966
- 出版者・発行元
- Anticancer Research USA Inc.
Background/Aim: Chitosan-coated iron oxide nanoparticles (Chi-NP) have gained attention because of their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. In this study, we investigated various biological properties of Chi-NP. Materials and Methods: ChiNP was prepared by mixing magnetic NP with chitosan FL-80. Particle size was determined by scanning and transmission electron microscopes, cell viability by MTT assay, cell cycle distribution by cell sorter, synergism with anticancer drugs by combination index, PGE(2) production in human gingival fibroblast was assayed by ELISA. Results: The synthetic process of Chi-NP from FL-80 and magnetic NP increased the affinity to cells, up to the level attained by nanofibers. Upon contact with the culture medium, Chi-NP instantly formed aggregates and interfered with intracellular uptake. Aggregated Chi-NP did not show cytotoxicity, synergism with anticancer drugs, induce apoptosis (accumulation of subG1 cell population), protect the cells from X-ray-induced damage, nor affected both basal and IL-1 beta-induced PGE, production. Conclusion: Chi-NP is biologically inert and shows high affinity to cells, further confirming its superiority as a scaffold for drug delivery.
- リンク情報
- ID情報
-
- DOI : 10.21873/invivo.11966
- ISSN : 0258-851X
- eISSN : 1791-7549
- Web of Science ID : WOS:000548905200003