2013
R-loop formation in trans at an AGGAG repeat
Journal of Nucleic Acids
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- ,
- Volume
- 2013
- Number
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1155/2013/629218
Formation of RNA-DNA hybrid, or R-loop, was studied in vitro by transcribing an AGGAG repeat with T7 RNA polymerase. When ribonuclease T1 was present, R-loop formation in cis was diminished, indicating that the transcript was separated from the template and reassociated with it. The transcript was found to form an R-loop in trans with DNA comprising the AGGAG repeat, when the DNA was supercoiled. Results of chemical modification indicated that the duplex opened at the AGGAG repeat under negative supercoiling. © 2013 Kazuya Toriumi et al.
- Link information
- ID information
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- DOI : 10.1155/2013/629218
- ISSN : 2090-0201
- ISSN : 2090-021X
- SCOPUS ID : 84884270291