2008年10月
Molecular Structure and Properties of Lectin from Tomato Fruit
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 72
- 号
- 10
- 開始ページ
- 2640
- 終了ページ
- 2650
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.80310
- 出版者・発行元
- TAYLOR & FRANCIS LTD
A cDNA encoding tomato fruit lectin was cloned from an unripe cherry-tomato fruit cDNA library. The isolated lectin cDNA contained an open reading frame encoding 365 amino acids, including peptides that were sequenced. The deduced sequence consisted of three distinct domains: (i) an N-terminal short extensin-like domain; (ii) a Cys-rich carbohydrate binding domain composed of four almost identical chitin-binding domains; (iii) an internal extensin-like domain of 101 residues containing 15 SerPro(4) motifs inserted between the first and second chitin-binding domains. The molecular weight of the lectin was 65,633 and that of the deglycosylated lectin was 32,948, as determined by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). This correlated with the estimated molecular weight of the deduced sequence. Recombinant tomato lectin expressed in Pichia pastoris possessed chitin-binding but not hemagglutinating activity. These findings confirmed that the cDNA encoded tomato lectin.
- リンク情報
- ID情報
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- DOI : 10.1271/bbb.80310
- ISSN : 0916-8451
- eISSN : 1347-6947
- Web of Science ID : WOS:000260619100019