MISC

2009年5月

Differential interaction and aggregation of 3-repeat and 4-repeat tau isoforms with 14-3-3 zeta protein

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Golam Sadik
  • ,
  • Toshihisa Tanaka
  • ,
  • Kiyoko Kato
  • ,
  • Kentaro Yanagi
  • ,
  • Takashi Kudo
  • ,
  • Masatoshi Takeda

383
1
開始ページ
37
終了ページ
41
記述言語
英語
掲載種別
DOI
10.1016/j.bbrc.2009.03.107
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Tau isoforms, 3-repeat (3R) and 4-repeat tau (4R), are differentially involved in neuronal development and in several tauopathies. 14-3-3 protein binds to tau and 14-3-3/tau association has been found both in the development and in tauopathies. To understand the role of 14-3-3 in the differential regulation of tau isoforms, we have performed Studies on the interaction and aggregation of 3R-tau and 4R-tau, either phosphorylated or unphosphorylated, with 14-3-3 zeta. We show by Surface plasmon resonance studies that the interaction between Unphosphorylated 3R-tau and 14-3-3 zeta is similar to 3-folds higher than that between unphosphorylated 4R-tau and 14-3-3 zeta. Phosphorylation of tau by Protein kinase A (PKA) increases the affinity of both 3R- and 4R-tau for 14-3-3 zeta to a similar level. An in vitro aggregation assay employing both transmission electron microscopy and fluorescence spectroscopy revealed the aggregation of unphosphorylated 4R-tau to be significantly higher than that of unphosphorylated 3R-tau following the induction of 14-3-3 zeta. The filaments formed from 3R- and 4R-tau were almost similar in morphology. In contrast, the aggregation of both 3R- and 4R-tau was reduced to a similar low level after phosphorylation with PKA. Taken together, these results suggest that 14-3-3 zeta exhibits a similar role for tau isoforms after PKA-phosphorylation, but a differential role for unphosphorylated tau. The significant aggregation of 4R-tau by 14-3-3 zeta suggests that 14-3-3 may act as an inducer in the generation of 4R-tau-predominant neurofibrillary tangles in tauopathies. (C) 2009 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 19

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2009.03.107
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000265768800008&DestApp=WOS_CPL

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