2011年1月
Microdialysis with radiometric monitoring of L-[beta-C-11]DOPA to assess dopaminergic metabolism: effect of inhibitors of L-amino acid decarboxylase, monoamine oxidase, and catechol-O-methyltransferase on rat striatal dialysate
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
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- 巻
- 31
- 号
- 1
- 開始ページ
- 124
- 終了ページ
- 131
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1038/jcbfm.2010.58
- 出版者・発行元
- NATURE PUBLISHING GROUP
The catecholamine, dopamine (DA), is synthesized from 3,4-dihydroxy-L-phenylalanine (L-DOPA) by aromatic L-amino acid decarboxylase (AADC). Dopamine metabolism is regulated by monoamine oxidase (MAO) and catechol-O-methyltransferase (COMT). To measure dopaminergic metabolism, we used microdialysis with radiometric detection to monitor L-[beta-C-11]DOPA metabolites in the extracellular space of the rat striatum. We also evaluated the effects of AADC, MAO, and COMT inhibitors on metabolite profiles. The major early species measured after administration of L-[beta-C-11]DOPA were [C-11]3,4-dihydroxyphenylacetic acid ([C-11]DOPAC) and [C-11]homovanillic acid ([C-11]HVA) in a 1:1 ratio, which shifted toward [C-11]HVA with time. An AADC inhibitor increased the uptake of L-[beta-C-11]DOPA and L-3-O-methyl-[C-11]DOPA and delayed the accumulation of [C-11]DOPAC and [C-11]HVA. The MAO and COMT inhibitors increased the production of [C-11]3-methoxytyramine and [C-11] DOPAC, respectively. These results reflect the L-DOPA metabolic pathway, suggesting that this method may be useful for assessing dopaminergic metabolism. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 124-131; doi:10.1038/jcbfm.2010.58; published online 21 April 2010
- リンク情報
- ID情報
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- DOI : 10.1038/jcbfm.2010.58
- ISSN : 0271-678X
- Web of Science ID : WOS:000285870700015