MISC

2011年1月

Microdialysis with radiometric monitoring of L-[beta-C-11]DOPA to assess dopaminergic metabolism: effect of inhibitors of L-amino acid decarboxylase, monoamine oxidase, and catechol-O-methyltransferase on rat striatal dialysate

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
  • Maki Okada
  • ,
  • Ryuji Nakao
  • ,
  • Rie Hosoi
  • ,
  • Ming-Rong Zhang
  • ,
  • Toshimitsu Fukumura
  • ,
  • Kazutoshi Suzuki
  • ,
  • Osamu Inoue

31
1
開始ページ
124
終了ページ
131
記述言語
英語
掲載種別
DOI
10.1038/jcbfm.2010.58
出版者・発行元
NATURE PUBLISHING GROUP

The catecholamine, dopamine (DA), is synthesized from 3,4-dihydroxy-L-phenylalanine (L-DOPA) by aromatic L-amino acid decarboxylase (AADC). Dopamine metabolism is regulated by monoamine oxidase (MAO) and catechol-O-methyltransferase (COMT). To measure dopaminergic metabolism, we used microdialysis with radiometric detection to monitor L-[beta-C-11]DOPA metabolites in the extracellular space of the rat striatum. We also evaluated the effects of AADC, MAO, and COMT inhibitors on metabolite profiles. The major early species measured after administration of L-[beta-C-11]DOPA were [C-11]3,4-dihydroxyphenylacetic acid ([C-11]DOPAC) and [C-11]homovanillic acid ([C-11]HVA) in a 1:1 ratio, which shifted toward [C-11]HVA with time. An AADC inhibitor increased the uptake of L-[beta-C-11]DOPA and L-3-O-methyl-[C-11]DOPA and delayed the accumulation of [C-11]DOPAC and [C-11]HVA. The MAO and COMT inhibitors increased the production of [C-11]3-methoxytyramine and [C-11] DOPAC, respectively. These results reflect the L-DOPA metabolic pathway, suggesting that this method may be useful for assessing dopaminergic metabolism. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 124-131; doi:10.1038/jcbfm.2010.58; published online 21 April 2010

リンク情報
DOI
https://doi.org/10.1038/jcbfm.2010.58
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000285870700015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/jcbfm.2010.58
  • ISSN : 0271-678X
  • Web of Science ID : WOS:000285870700015

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