2016年4月
Cryptic antifungal compounds active by synergism with polyene antibiotics
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
- ,
- ,
- ,
- 巻
- 121
- 号
- 4
- 開始ページ
- 394
- 終了ページ
- 398
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jbiosc.2015.08.003
- 出版者・発行元
- SOC BIOSCIENCE BIOENGINEERING JAPAN
The majority of antifungal compounds reported so far target the cell wall or cell membrane of fungi, suggesting that other types of antibiotics cannot exert their activity because they cannot penetrate into the cells. Therefore, if the permeability of the cell membrane could be enhanced, many antibiotics might be found to have antifungal activity. We here used the polyene antibiotic nystatin, which binds to ergosterol and forms pores at the cell membrane, to enhance the cellular permeability. In the presence of nystatin, many culture extracts from entomopathogenic fungi displayed antifungal activity. Among all the active extracts, two active components were purified and identified as helvolic acid and terramide A. Because the minimum inhibitory concentration of either compound was reduced four-fold in the presence of nystatin, it can be concluded that this screening method is useful for detecting novel antifungal activity. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.jbiosc.2015.08.003
- ISSN : 1389-1723
- eISSN : 1347-4421
- Web of Science ID : WOS:000375161900007