論文

査読有り
1997年5月

Developmental regulation, expression, and apoptotic potential of galectin-9, a beta-galactoside binding lectin

JOURNAL OF CLINICAL INVESTIGATION
  • J Wada
  • ,
  • K Ota
  • ,
  • A Kumar
  • ,
  • EI Wallner
  • ,
  • YS Kanwar

99
10
開始ページ
2452
終了ページ
2461
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1172/jci119429
出版者・発行元
ROCKEFELLER UNIV PRESS

Galectin-9, a beta-galactoside binding lectin, has recently been isolated from murine embryonic kidney. In this study, its biological functions and expression in embryonic, newborn, and adult mice tissues were investigated. By Northern blot analyses, it was found widely distributed and its expression was developmentally regulated. In situ hybridization studies revealed an accentuated expression of galectin-9 in liver and thymus of embryonic mice. In postnatal mice, antigalectin-9 immunoreactivity was observed in various tissues, including thymic epithelial cells. The high expression of galectin-9 in the thymus led us to investigate its role in the clonal deletion of thymocytes. Fusion proteins were generated, which retained lactose-binding activity like the endogenous galectin-9. Galectin-9, at 2.5 mu M concentration, induced apoptosis in similar to 30% of the thymocytes, as assessed by terminal deoxytransferase-mediated dUTP nick end labeling method. The apoptotic effect was dose dependent and lactose inhibitable. At higher concentrations, it induced homotypic aggregation of the thymocytes. Electron microscopy revealed similar to 60% of the thymocytes undergoing apoptosis in the presence of galectin-9. By immunofluorescence microscopy, some of the thymocytes undergoing apoptosis had plasmalemmal bound galectin-9. Galectin-9 failed to induce apoptosis in hepatocytes. Taken together, these findings indicate that galectin-9, a developmentally regulated lectin, plays a role in thymocyte-epithelial interactions relevant to the biology of the thymus.

Web of Science ® 被引用回数 : 238

リンク情報
DOI
https://doi.org/10.1172/jci119429
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1997XB22600025&DestApp=WOS_CPL
URL
http://orcid.org/0000-0003-1468-5170
ID情報
  • DOI : 10.1172/jci119429
  • ISSN : 0021-9738
  • ORCIDのPut Code : 17913173
  • Web of Science ID : WOS:A1997XB22600025

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