論文

査読有り
1996年12月

D-glucose-induced dysmorphogenesis of embryonic kidney

JOURNAL OF CLINICAL INVESTIGATION
  • YS Kanwar
  • ,
  • ZZ Liu
  • ,
  • A Kumar
  • ,
  • MI Usman
  • ,
  • J Wada
  • ,
  • EI Wallner

98
11
開始ページ
2478
終了ページ
2488
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
ROCKEFELLER UNIV PRESS

An organ culture system was used to study the effect of D-glucose on embryonic kidneys, and to delineate the mechanism(s) relevant to their dysmorphogenesis. Metanephroi were cultured in the presence of 30 mM D-glucose. A notable reduction in the size and population of nephrons was observed. Ureteric bud branches were rudimentary and the acuteness of their tips, the site of nascent nephron formation, was lost. Metanephric mesenchyme was atrophic, had reduced cell replication, and contained numerous apoptotic cells. Competitive reverse transcriptase-PCR analyses and immunoprecipitation studies indicated a decrease in expression of heparan sulfate proteoglycan (perlecan). Status of activated protein-2 was evaluated since its binding moths are present in the promoter region of the perlecan gene. Decreased binding activity of activated protein-5 related to its phosphorylation, was observed. D-glucose-treated explants also had reduced levels of cellular ATP. Exogenous administration of ATP restored the altered metanephric morphology and reduced [S-35]sulfate-incorporated radioactivity associated with perlecan. The data suggest that D-glucose adversely affects the metanephrogenesis by perturbing various cellular phosphorylation events involved in the transcriptional and translational regulation of perlecan. Since perlecan modulates epithelial/mesenchymal interactions, its deficiency may have led to the metanephric dysmorphogenesis and consequential atrophy of the mesenchyme exhibiting accelerated apoptosis.

Web of Science ® 被引用回数 : 49

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1996VX29100010&DestApp=WOS_CPL
URL
http://orcid.org/0000-0003-1468-5170
ID情報
  • ISSN : 0021-9738
  • ORCIDのPut Code : 17913179
  • Web of Science ID : WOS:A1996VX29100010

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