論文

査読有り
2017年10月

Practical Liposomal Formulation for Taxanes with Polyethoxylated Castor Oil and Ethanol with Complete Encapsulation Efficiency and High Loading Efficiency

NANOMATERIALS
  • Tsukasa Shigehiro
  • ,
  • Junko Masuda
  • ,
  • Shoki Saito
  • ,
  • Apriliana C. Khayrani
  • ,
  • Kazumasa Jinno
  • ,
  • Akimasa Seno
  • ,
  • Arun Vaidyanath
  • ,
  • Akifumi Mizutani
  • ,
  • Tomonari Kasai
  • ,
  • Hiroshi Murakami
  • ,
  • Ayano Satoh
  • ,
  • Tetsuya Ito
  • ,
  • Hiroki Hamada
  • ,
  • Yuhki Seno
  • ,
  • Tadakatsu Mandai
  • ,
  • Masaharu Seno

7
10
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/nano7100290
出版者・発行元
MDPI AG

Taxanes including paclitaxel and docetaxel are effective anticancer agents preferably sufficient for liposomal drug delivery. However, the encapsulation of these drugs with effective amounts into conventional liposomes is difficult due to their high hydrophobicity. Therefore, an effective encapsulation strategy for liposomal taxanes has been eagerly anticipated. In this study, the mixture of polyethoxylated castor oil (Cremophor EL) and ethanol containing phosphate buffered saline termed as CEP was employed as a solvent of the inner hydrophilic core of liposomes where taxanes should be incorporated. Docetaxel-, paclitaxel-, or 7-oxacetylglycosylated paclitaxel-encapsulating liposomes were successfully prepared with almost 100% of encapsulation efficiency and 29.9, 15.4, or 29.1 mol% of loading efficiency, respectively. We then applied the docetaxel-encapsulating liposomes for targeted drug delivery. Docetaxel-encapsulating liposomes were successfully developed HER2-targeted drug delivery by coupling HER2-specific binding peptide on liposome surface. The HER2-targeting liposomes exhibited HER2-specific internalization and enhanced anticancer activity in vitro. Therefore, we propose the sophisticated preparation of liposomal taxanes using CEP as a promising formulation for effective cancer therapies.


リンク情報
DOI
https://doi.org/10.3390/nano7100290
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28946623
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000414916100008&DestApp=WOS_CPL