1995年11月
DISTINCT SIGNAL-TRANSDUCTION THROUGH THE TYROSINE-CONTAINING DOMAINS OF THE GRANULOCYTE-COLONY-STIMULATING FACTOR-RECEPTOR
EMBO JOURNAL
- ,
- ,
- 巻
- 14
- 号
- 21
- 開始ページ
- 5288
- 終了ページ
- 5296
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- OXFORD UNIV PRESS UNITED KINGDOM
The receptor for granulocyte colony-stimulating factor (G-CSFR) is a hemopoietic growth factor receptor, which mediates proliferation and differentiation signals. The cytoplasmic region of G-CSFR carries four tyrosine residues in its C-terminal half. We constructed mutant receptors in which each tyrosine residue of G-CSFR was mutated to phenylalanine. Two mutant receptors (Tyr703 and Tyr728) neither transduced the growth-inhibitory signal nor induced the neutrophil-specific myeloperoxidase (MPO) gene. The Tyr703 mutant did not induce morphological changes in cells, whereas transformants expressing the Tyr728 mutant adhered to plates with a macrophage-like morphology upon G-CSF stimulation. Mutation of the most distal tyrosine residue (Tyr763) abolished the ability of G-CSFR to stimulate the tyrosine phosphorylation of a cellular protein with an M(r) of 54 kDa. These results indicated that the regions around the three tyrosine residues of G-CSFR play essential and distinct roles in signal transduction.
- リンク情報
- ID情報
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- ISSN : 0261-4189
- Web of Science ID : WOS:A1995TF68100015