論文

査読有り
2004年4月

Specific recruitment of SPA-1 to the immunological synapse: involvement of actin-bundling protein actinin

IMMUNOLOGY LETTERS
  • M Harazaki
  • ,
  • Y Kawai
  • ,
  • L Su
  • ,
  • Y Hamazaki
  • ,
  • T Nakahata
  • ,
  • N Minato
  • ,
  • M Hattori

92
3
開始ページ
221
終了ページ
226
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.imlet.2004.01.004
出版者・発行元
ELSEVIER SCIENCE BV

SPA-1 is involved in the regulation of T cell activation in response to antigens through the control of Rap I GTPase signaling. In this study, the subcellular localization of SPA-1 in the T cells was examined by using anti-SPA-1 antibody and GFP-SPA-1. While SPA-I was detected diffusely at the surface cortical region in the floating unpolarized T cells, it was concentrated at the matrix-adhesion region with dense actin-cytoskeleton. Upon interaction with specific antigen-presenting cells, SPA-1 was highly concentrated at the immunological synapse closely co-localizing with actin. By yeast two-hybrid system, SPA-1 was shown to interact with an actin-bundling protein alpha-actinin, and it was indicated that SPA-1 co-localized with alpha-actinin at the immunological synapse. The results have suggested that SPA-1 in the T cells is selectively recruited to the immunological synapse with dense actin-cytoskeletal reorganization and keeps restraining the levels of Rap1GTP at the local TCR-signaling complex for the T cell activation. (C) 2004 Elsevier B.V. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.imlet.2004.01.004
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201302262865115600
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15081616
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000221166600005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.imlet.2004.01.004
  • ISSN : 0165-2478
  • J-Global ID : 201302262865115600
  • PubMed ID : 15081616
  • Web of Science ID : WOS:000221166600005

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