2015年4月
Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription
EUROPEAN JOURNAL OF IMMUNOLOGY
- 巻
- 45
- 号
- 4
- 開始ページ
- 975
- 終了ページ
- 987
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/eji.201445295
- 出版者・発行元
- WILEY-BLACKWELL
It is believed that memory CD8(+) Tcells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation. Their survival has been shown to be dependent on IL-7, but it is unclear where they acquire it. Here we show that in murine BM, memory CD8(+) Tcells individually colocalize with IL-7(+) reticular stromal cells. The Tcells are resting in terms of global transcription and do not express markers of activation, for example, 4-1BB (CD137), IL-2, or IFN-, despite the expression of CD69 on about 30% of the cells. Ninety-five percent of the memory CD8(+) Tcells in BM are in G(0) phase of cell cycle and do not express Ki-67. Less than 1% is in S/M/G(2) of cell cycle, according to propidium iodide staining. While previous publications have estimated the extent of proliferation of CD8(+) memory Tcells on the basis of BrdU incorporation, we show here that BrdU itself induces proliferation of CD8(+) memory Tcells. Taken together, the present results suggest that CD8(+) memory Tcells are maintained as resting cells in the BM in dedicated niches with their survival conditional on IL-7 receptor signaling.
- リンク情報
- ID情報
-
- DOI : 10.1002/eji.201445295
- ISSN : 0014-2980
- eISSN : 1521-4141
- Web of Science ID : WOS:000352543300005