論文

査読有り 国際誌
2019年10月16日

Mesenchymal stromal cells in bone marrow express adiponectin and are efficiently targeted by an adiponectin promoter-driven Cre transgene.

International immunology
  • Hisa Mukohira
  • ,
  • Takahiro Hara
  • ,
  • Shinya Abe
  • ,
  • Shizue Tani-Ichi
  • ,
  • Atsuko Sehara-Fujisawa
  • ,
  • Takashi Nagasawa
  • ,
  • Kazuyuki Tobe
  • ,
  • Koichi Ikuta

31
11
開始ページ
729
終了ページ
742
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/intimm/dxz042

Stromal cells in bone marrow (BM) constitute a specific microenvironment supporting the development and maintenance of hematopoietic cells. Adiponectin is a cytokine secreted by adipocytes. Besides its anti-diabetic and anti-atherogenic roles, adiponectin reportedly regulates the development and function of hematopoietic cells in BM. However, it remains unclear whether mesenchymal stromal cells in BM express adiponectin. Here, we show that PDGFRβ+VCAM-1+ stromal cells express adiponectin. Lineage tracing revealed that a majority of PDGFRβ+VCAM-1+ cells were targeted by an adiponectin promoter-driven Cre (Adipoq-Cre) transgene. Additionally, the Adipoq-Cre transgene targets a minority of osteoblasts at a younger age but larger populations are targeted at an older age. Furthermore, the Adipoq-Cre transgene targets almost all CXCL12-abundant reticular (CAR) cells and most of the stromal cells targeted by the Adipoq-Cre transgene are CAR cells. Finally, deletion of interleukin-7 (IL-7) by the Adipoq-Cre transgene resulted in severe impairment of B lymphopoiesis in BM. These results demonstrate that PDGFRβ+VCAM-1+ stromal cells in BM express adiponectin and are targeted by the Adipoq-Cre transgene, suggesting a broader specificity of the Adipoq-Cre transgene.

リンク情報
DOI
https://doi.org/10.1093/intimm/dxz042
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31094421
ID情報
  • DOI : 10.1093/intimm/dxz042
  • PubMed ID : 31094421

エクスポート
BibTeX RIS