1996年12月
The V-J recombination of T cell receptor-gamma genes is blocked in interleukin-7 receptor-deficient mice
JOURNAL OF EXPERIMENTAL MEDICINE
- ,
- ,
- 巻
- 184
- 号
- 6
- 開始ページ
- 2423
- 終了ページ
- 2427
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1084/jem.184.6.2423
- 出版者・発行元
- ROCKEFELLER UNIV PRESS
IL-7R-deficient mice have severely impaired expansion of early lymphocytes and lack gamma delta T cells. To elucidate the role of IL-7R on gamma delta T cell development, we analyzed the rearrangements of TCR-alpha, beta, gamma, and delta genes in the thymus of the IL-7R-deficient mice. Southern blot analysis with a J gamma 1 probe revealed that more than 70% of J gamma 1 and J gamma 2 alleles are recombined to form distinct V gamma 1.2-J gamma 2 and V gamma 2-J gamma 1 fragments in control mice. On the contrary, no such recombination was detected in the mutant mice. The rearrangements in the TCR-alpha, beta, and delta loci were comparably observed in control and mutant mice. PCR analysis indicated that the V-J recombination of all the V gamma genes is severely hampered in the mutant mice. The mRNA of RAG-1, RAG-2, Ku-80, and terminal deoxynucleotidyl transferase (TdT) genes was equally detected between control and mutant thymi, suggesting that the expression of common recombination machinery is not affected. These data demonstrated that the V-J recombination of the TCR gamma genes is specifically blocked in the IL-7R-deficient mice and suggested the presence of highly specific regulation for TCR gamma gene rearrangement.
- リンク情報
- ID情報
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- DOI : 10.1084/jem.184.6.2423
- ISSN : 0022-1007
- Web of Science ID : WOS:A1996VZ41300036