論文

査読有り
2002年12月

Impact of the p53 status of the tumor cells on the effect of reactor neutron beam irradiation, with emphasis on the response of intratumor quiescent cells

JAPANESE JOURNAL OF CANCER RESEARCH
  • S Masunaga
  • ,
  • K Ono
  • ,
  • A Takahashi
  • ,
  • Y Sakurai
  • ,
  • K Ohnishi
  • ,
  • T Kobayashi
  • ,
  • Y Kinashi
  • ,
  • M Takagaki
  • ,
  • T Ohnishi

93
12
開始ページ
1366
終了ページ
1377
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
BUSINESS CENTER ACADEMIC SOCIETIES JAPAN

Human head and neck squamous cell carcinoma cells transfected with mutant p53 (SAS/mp53) or with neo vector as a control (SAS/neo) were inoculated subcutaneously into both the hind legs of Balb/cA nude mice. Tumor-bearing mice received 5-bromo-2'-deoxyuridine (BrdU) continuously to label all proliferating (P) cells in the tumors. After administration of sodium borocaptate-B-10 (BSH) or p-boronophenylalanine-B-10 (BPA), the tumors were irradiated with neutron beams. The tumors not treated with B-10-compound were irradiated with neutron beams or gamma-rays. The tumors were then excised, minced and trypsinized. The tumor cell suspensions thus obtained were incubated with a cytokinesis blocker, and the micronucleus (MN) frequency in cells without BrdU labeling (=quiescent (Q) cells) was determined using immunofluorescence staining for BrdU. Meanwhile, 6 h after irradiation, tumor cell suspensions obtained in the same manner were used for determining the frequency of apoptosis in Q cells. The MN and apoptosis frequencies in total (P+Q) tumor cells were determined from the tumors that were not pretreated with BrdU. Without B-10-carriers, in both tumors, the relative biological effectiveness of neutrons was greater in Q cells than in total cells, and larger for low than high cadmium ratio neutrons. With B-10-carriers, the sensitivity was increased for each cell population, especially for total cells. BPA increased both frequencies for total cells more than BSH. Nevertheless, the sensitivity of Q cells treated with BPA was lower than that of BSH-treated Q cells. These sensitization patterns in combination with B-10-carriers were clearer in SAS/neo than in SAS/mp53 tumors. The p53 status of the tumor cells had the potential to affect the response to reactor neutron beam irradiation following B-10-carrier administration.

リンク情報
CiNii Articles
http://ci.nii.ac.jp/naid/50000713755
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12495477
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000180005700012&DestApp=WOS_CPL
ID情報
  • ISSN : 0910-5050
  • CiNii Articles ID : 50000713755
  • PubMed ID : 12495477
  • Web of Science ID : WOS:000180005700012

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