Detailed analysis of myocardial deformation through a whole cardiac cycle was accomplished using a tagging method with a high-density grid. Four sets of tagged images with a 4-mm-spacing grid were measured by generating four tagging pulses arranged at regular intervals in the cardiac cycle. Through each set of images, tag intersections were tracked semi-automatically. The estimated motions of tag intersections were concatenated so that sequential positions of myocardium were connected through a whole cardiac cycle. In vitro evaluation of the precision of this technique showed that the mean error of tracked 4-mm tag intersections was less than 0.47 +/- 0.17 mm, even on the quite low-contrast images, and the concatenation error caused by double concatenation was comparable to the interpolation error in the subendocardial area obtained with 8-mm tag intersection motion. The small difference between the two mean distance curves of the in vivo evaluation indicated that the method is useful for analyzing heart wall abnormalities. (C) 2000 Wiley-Liss, Inc.