NAKABAYASHI Hidekazu

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Name
NAKABAYASHI Hidekazu
Affiliation
Hokkaido Chitose College of Rehabilitation
Section
Faculty of Health Science
Job title
Professor
Degree
Doctor of Medical Science(Okayama University)

Research Areas

 
 

Academic & Professional Experience

 
 
   
 
Research Associate at Department of Biochemistry,
 
 
   
 
Hokkaido Unuversity School of Medicine
 
1973
 - 
1987
Research Associate at the Division of Pathology, Cancer Institute, Okayama University Medical School
 
1975
 - 
1985
Lecturer at the School of Clinical Inspector, Okayama University
 
1983
 - 
1987
Lecturer at the Postgraduate School, Okayama University Medical School
 
1985
 - 
1986
Post-doctoral fellow of Alberta Heritage Foundation at the Oncology Research Group, Department of Medical Biochemistry, Faculty of Medicine, University of Calgary
 
1988
 - 
1991
Post-doctoral fellow at the Oncology Research Group, Department of Medical Biochemistry, Faculty of Medicine, University of Calgary
 
1991
 - 
1995
Visiting Scientist at the Oncology Research Group, Department of Medical Biochemistry, Faculty of Medicine, University of Calgary
 
1991
 - 
1997
Research director at Japan Immunoresearch Laboratories Co. Ltd, Takasaki, Gunma, Japan
 
1992
 - 
1997
Adjunct Lecturer at Department of Public Health, Okayama University Medical School
 
1997
 - 
Today
 

Education

 
 
 - 
1973
Faculty of Science, Okayama University
 

Awards & Honors

 
1985
Post-doctoral fellow of Alberta Heritage Foundation
 
 
Alberta Heritage Foundation of Medical Research, Alberta Heritage Foundation
 

Published Papers

 
Sakata N, Kaneko S, Ikeno S, Miura Y, Nakabayashi H, Dong XY, Dong JT, Tamaoki T, Nakano N, Itoh S
Journal of signal transduction   2014 970346   2014   [Refereed]
Nakabayashi Hidekazu, Taketa Kazuhisa
Okayama Igakkai Zasshi (Journal of Okayama Medical Association)   124(3) 231-238   Dec 2012
高分化型ヒト肝癌由来細胞株"HuH-7"は,1982年にCancer Researchにその樹立を報告した.HuH-7は,当時の岡山大学医学部附属癌源研究施設病理部門(故佐藤二郎教授)の下で樹立し,これまで多くの研究分野で利用され,世界的に有名な肝癌細胞株となっている.本稿では,有用性の高い分化機能を有するヒト肝癌細胞株HuH-7について,肝細胞癌の腫瘍マーカーであるα-fetoprotein(AFP)を中心に,この細胞株を用いた研究分野に関する詳細を紹介する.
The changes of Cyclopedic Glycan Patterns during the Process of Carcinogenesis
Fujita Hiroko, Ogawa Tomohisa, Takeishi Shunsaku, Yamada Masao, Nakabayashi Hidekazu
GLYCOBIOLOGY   18(11) 958   Nov 2008   [Refereed]
Li HM, Ikeda H, Nakabayashi H, Nishi S, Sakai M
Gene   389(2) 128-135   Mar 2007   [Refereed]
Dong J, Fujii S, Li H, Nakabayashi H, Sakai M, Nishi S, Goto D, Furumoto T, Imagawa S, Zaman TA, Kitabatake A
Arteriosclerosis, thrombosis, and vascular biology   25 1078-1084   May 2005   [Refereed]
Nakabayashi H, Koyama Y, Suzuki H, Li HM, Sakai M, Miura Y, Wong NC, Nishi S
Biochemical and biophysical research communications   318(3) 773-785   Jun 2004   [Refereed]
ING1 represses transcription by direct DNA binding and through effects on p53.
Kataoka H, Bonnefin P, Vieyra D, Feng X, Hara Y, Miura Y, Joh T, Nakabayashi H, Vaziri H, Harris CC, Riabowol K
Cancer research   63 5785-5792   Sep 2003   [Refereed]
Nakabayashi H, Koyama Y, Sakai M, Li HM, Wong NC, Nishi S
Biochemical and biophysical research communications   287(1) 160-172   Sep 2001   [Refereed]
Kataoka H, Miura Y, Joh T, Seno K, Tada T, Tamaoki T, Nakabayashi H, Kawaguchi M, Asai K, Kato T, Itoh M
Oncogene   20(7) 869-873   Feb 2001   [Refereed]
Aneuploidy of sex chromosomes in basal cell carcinoma : Its clonality and involvement in the development of carcinogenesis
Into.J. Oncol.      2000
Huang, K., Serria, M.S., Nakabayashi, H., Nishi, S., and Sakai, M.
Gene      2000
The Maf family of the transcription factors plays a pivotal role in controlling development and cellular differentiation. To clarify the molecular mechanisms controlling mafB expression, a genomic clone of the mouse mafB gene was isolated and anal...
Sato Y, Tanaka K, Lee G, Kanegae Y, Sakai Y, Kaneko S, Nakabayashi H, Tamaoki T, Saito I
Biochemical and biophysical research communications   244(2) 455-462   Mar 1998   [Refereed]
In vivo gene therapy for alpha-fetoprotein-producing hepatocellular carcinoma by adenovirus-mediated transfer of cytosine deaminase gene.
Kanai F, Lan KH, Shiratori Y, Tanaka T, Ohashi M, Okudaira T, Yoshida Y, Wakimoto H, Hamada H, Nakabayashi H, Tamaoki T, Omata M
Cancer research   57 461-465   Feb 1997   [Refereed]
In vivo gene therapy for α-fetoprotein-producing hepatocelluvar carcinoma by adenovirus-mediated transter of cytosine deaminase gene
Cancer Res.   57 461-465   1997
[Regulation of tumor marker gene expression].
Sakai M, Nakabayashi H
Nihon rinsho. Japanese journal of clinical medicine   54(6) 1560-1567   Jun 1996   [Refereed]
Kanai F, Shiratori Y, Yoshida Y, Wakimoto H, Hamada H, Kanegae Y, Saito I, Nakabayashi H, Tamaoki T, Tanaka T, Lan KH, Kato N, Shiina S, Omata M
Hepatology (Baltimore, Md.)   23(6) 1359-1368   Jun 1996   [Refereed]
Adenovirus-mediated gene therapy of hepatocellular carcinoma using cancer-specific gene expression.
Kaneko S, Hallenbeck P, Kotani T, Nakabayashi H, McGarrity G, Tamaoki T, Anderson WF, Chiang YL
Cancer research   55 5283-5287   Nov 1995   [Refereed]
Most patients with hepatocellular carcinoma have an elevated α-fetoprotein (AFP) level. This high level of AFP expression is transcriptionally controlled by the 5′-flanking sequence of the AFP gene. Using the 5′-flanking sequence as a promoter for...
Cell-specific and non-cell-specific enhancer activity in a far upstream region of the human alpha-fetoprotein gene.
Nakabayashi,H., Suzuki,H., Tamaoki,T.
肝臓   36(Suppl.1) 219-219   Jun 1995
Gene therapy for human hepatoma cells by adenovirus vector
   1995
Down-regulation of human α-fetoprotein gene by C-jun ad C-fos
Saegusa, M., Ido, A., Nakabayashi, H., and Tamaoki, T.
J. Tumor Marker Oncol.   9 29-34   1994
Effects of jun and fos genes on expression of the human α-fetoprotein(AFP)gene was studied by transfecting a jun or fos expression plasmid into HuH-7 human hepatoma cells with the chloramphenicol acetyltransferase(CAT) gene linked to the human AFP...
Namba Masayoshi, Nakabayashi Hidekazu, Doi Isao, Sato Jiro, Miyazaki Masahiro
TISSUE CULTURE RESEARCH COMMUNICATIONS   12(3) 221-227   1993
Cultured cells have played an important role in the recent remarkable progress of cell biology. Many studies on monoclonal antibodies, oncogenes, cytogenetics, growth factors, and cytokines including interferons, have been carried out with culture...
Nucleic Acid Res.   21(5) 1205-1211   1993
Cellular characteristics and utilization of human hepatoma cell lines which were established in our laboratoy and distributed by Japanese cancer research
Tissue Culture Res. Commun.   12 221-227   1993
Identification and characterization of two enhancers of the human albumin gene.
Hayashi Y, Chan J, Nakabayashi H, Hashimoto T, Tamaoki T
The Journal of biological chemistry   267 14580-14585   Jul 1992   [Refereed]
A possible mechanism of inverse developmental regulation of alpha-fetoprotein and albumin genes. Studies with epidermal growth factor and phorbol ester.
Nakata K, Motomura M, Nakabayashi H, Ido A, Tamaoki T
The Journal of biological chemistry   267 1331-1334   Jan 1992   [Refereed]
A possible mechanism of inverse developmental regulation of α-fetoprotein and albumin genes
J. Biol. Chem.   267 1331-1334   1992
Selective expression of a toxin gene under the direction of human α-fetoprotein gene regulatory sequences in the human hepatomn cells-An approach to hepatocellular carcinoma gene therapy
Human gene therapy   3 614   1992
A position-dependent silencer plays a major role in repressing α-fetoprotein expression -A possible mechanisms for deveropmental change of α-fetoprotein and albumin genes
Dept. of Med. Biochem 8th Annual Meeting      1992
A position-dependent silencer plays a major role in repressing alpha-fetoprotein expression in human hepatoma.
Nakabayashi H, Hashimoto T, Miyao Y, Tjong KK, Chan J, Tamaoki T
Molecular and cellular biology   11 5885-5893   Dec 1991   [Refereed]
A position-dependent silencer plays a major role in repressing α-fetoprotein expression in human hepatoma
Mol. Cell. Biol.   11 5885-5893   1991
Cis-regulatory elements which suppress gene expression of α-fetoprotein
   1991
Relationship between cell growth/cell cycle and RB gene expression
   1991
c-Ha-ras down regulates the alpha-fetoprotein gene but not the albumin gene in human hepatoma cells.
Nakao K, Lawless D, Ohe Y, Miyao Y, Nakabayashi H, Kamiya H, Miura K, Ohtsuka E, Tamaoki T
Molecular and cellular biology   10 1461-1469   Apr 1990   [Refereed]
Transcriptional regulation of alpha-fetoprotein expression by dexamethasone in human hepatoma cells.
Nakabayashi H, Watanabe K, Saito A, Otsuru A, Sawadaishi K, Tamaoki T
The Journal of biological chemistry   264 266-271   Jan 1989   [Refereed]
Transcriptional regulation of α-fetoprotein expression by dexamethasone in human hepatoma cells
J. Biol. Chem.   264 266-271   1989
Establishment and characterization of the human osteosavcoma cell line
   1988
Tamaoki Taiki, Watanabe Kazutada, Nakabayashi Hidekazu
Gunma symposia on endocrinology   24 77-87   1987
Cis-acting elements involved in cell-specitic and hormonal regulation of the human α-fetoprotein gene
Gunma Symposia on Endocrinology   24 77-87   1987
Bovine serum-dependent alteration of lectin-reactive patterns of α-fetoprotein produced by HUH-6 Cl-5 cells
   1987
ヒト骨肉腫細胞に対するcis-Dichlorodiammineplatinumを併用した温熱療法について
Yamane Takashi, Nakabayashi Hidekazu, Ito Shiro
Hiroshima Journal of Medical Sciences   35(3) 191-195   Sep 1986
骨肉腫患者から採取したヒト骨肉腫細胞(HuO-3NI)と正常細胞(HuO-3)を組織培養し,これに対する温熱治療とcis-dichlorodiammineplatinum (CDDP)との併用療法につき検討した.HuO-3細胞は41°Cと42°Cで培養した所,細胞の増殖を認めたが,HuO-3NI細胞は著しく数が減った.CDDPとの併用療法では,37°CではHuO-3N1細胞の方がHuO-3細胞より著しく分裂したが,41°C又は42°CではHuO-3細胞が分裂可能な状態でも,分裂しなかった....
HATAHARA Terumasa, IGARASHI Shogo, FUNAKI Naoya, NAKABAYASHI Hidekazu, SATO Jiro
Kanzo   27(9) 1290-1295   1986
One cell line from human hepatoma, PLC/PRF/5, was found to produce type III procollagen, while four other ones, huH-1, huH-4, Huh-6c15 and Huh-7, were not. For the assay of aminoterminal peptide of type III procollagen, a radioimmunoassay was used...
In vitro thermochemotherapy of human osteosarcoma cells with cis-dichlorodiammineplatium(II)
Hiroshima J. Med. Sci.   35 191-195   1986
A glucocorticoid-responsive element of the human α-fetoprotein gene
6th Annual Heritage Medical Research Day      1986
Hormonal control of alpha-fetoprotein secretion in human hepatoma cell lines proliferating in chemically defined medium.
Nakabayashi H, Taketa K, Yamane T, Oda M, Sato J
Cancer research   45 6379-6383   Dec 1985   [Refereed]
Hormonal control of α-fetoprotein secretion in human hepatoma cell lines proliterating in chemically defined medium
Cancer Res.   45 6379-6383   1985
Further resolution of human α-fetoprotein by affinity electropho resis with erythroagglutinating phyto hemagglutinin of Phaseolus valgaris lectin
Tumor Biology   6 519-531   1985
In vitro chacacterization of cells devived from human giant-cell tumor of bone. -Chromosonal Changes-
28 345-348   1985
In vitro thermochemotherapy of human osteosarcoma cells with cis-dichlorodiammineplatium(II)
   1985
Microheterogeneity of human α-fetoprotein produced by cultured cell lines of hepatocellular carcinoma.
   1985
Gene expression of the human α-fetoprotein
Dept. of Med. Biochem 1st Annual Meeting      1985
Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.
Nakabayashi H, Taketa K, Yamane T, Miyazaki M, Miyano K, Sato J
Gann = Gan   75 151-158   Feb 1984   [Refereed]
Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically diffined medium
(Japan. J. Cancer Res.)   75 151-158   1984
Intestinal-type alkaline phosphatase produced by human hepatoblastoma cell line HUH-6 Clone5
Cancer Res.   44 339-344   1984
Production of procollagen peptide typeIII in human hepatoma cell line
25 1617   1984
Establishment and characterization of clonal cell lines derived from a human osteosarcoma
27 1610-1612   1984
Effect of purified AFP antibody on the growth of AFP producing cells derived from human hepatoma
   1984
Long-tern culture of hepatoma cells with differenitiated liver function in chemically defined medium
   1983
Effect of various hormons on the α-fetoprotein in human hepatoma cells proliferating in chemically defined medium
   1983
Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.
Nakabayashi H, Taketa K, Miyano K, Yamane T, Sato J
Cancer research   42 3858-3863   Sep 1982   [Refereed]
Effect of 3'-methyl-4-dimethylaminoazobenzene on liver cells from adult rat in primary culture.
Miyazaki, M., Wahid, S., Matsuura, K., Tsunashima, M., Nakabayashi, H., Miyano, K., and Sato, J.
Res. Exp. Med. (Berl.),   181 27-38   1982   [Refereed]
Bile acid synthesis by long-term cultured cell line established from a human hepatoblastoma
J. Clin. Inverst.   70 1128-1130   1982
Effect of 3'-methyl-4-dimethyl aminoazobenzene on livre cells from adult rat in primary culture
Res. Exp Med. (Berl. )   181 27-38   1982
Cultivation of the human hepatoma cell lines
18 274-280   1982
Growth of human hepatoma all lines with differentiated function in chemically defined medium.
Hidekazu Nakabayashi, Kazuhisa Taketa, Keiko Miyano, Takashi Yamane, and Jiro Sato
Cancer Res.   42 3858-3863   1982
  A chemically defined medium containing selenium was further improved, and the improved medium showed excellent ability to support growth and colony formation of a human hepatoma cell line, HuH-7. The improved defined medium (designated as IS-RPM...
Primary culture and cloning of human embryonic liver cells
   1982
Spontaneous neoplastic transformation in vitro of a diploid clone of rat liver cells under condition of large inoculum size of cells at the transter
Japan. J. Cancer Res.   72 377-387   1981
Culfivation of human liver cells : Growth and maintenance of cells in vitro
   1981
γ-Glutamyltransferase isozymes in the medium of cultured human hepatoma cell lines
   1981
The primary culture and maintenance of normal and malignant liver cells
112 156-159   1980
Growth and maintenance of epithelial cells derived from human enbryonec liver in uitro
   1980
Isolation and characterization of diploid clones from adult and new-born rat liver cell lines.
In Vitro   15 393-400   1979
NAKABAYASHI Hidekazu, SATO Jiro
Japan. J. Cancer Res.   68(1) 21-27   1977
In culture, a cloned rat liver cell line, J-5-2, exhibited a high diploidy for 200 days after the last cloning (diploid line) but, thereafter, pseudodiploid cells gradually increased in number (pseudodiploid line). These diploid and pseudodiploid ...
Effect of the inoculum size of cells on the maintenance of diploidy in cultured liver cell clones of the rat
   1977
Masuji Hiroshi, Nakabayashi Hidekazu, Sato Jiro
Acta Medica Okayama   28(4)    Aug 1974
<p>To obtain a useful rat liver cell line for in vitro carcinogenesis, two rat diploid epithelial cell lines were established from a 7-day-old male rat by the repeated colonial clone method. More than 80% of cells from each cell line have maintain...
Long-term cultivation of two diploid epithelial cell lines derived from rat liver cells
Acta Med. Okayama   28 289-293   1974

Misc

 
Cell-specific and non-cell-specific enhancer activity in a far upstream region of the human α-fetoprotein gene
31st Annual Meeting of Japan Society of Hepatology      1995

Books etc

 
Production and idenfification of different molecular species of human α-fetoprotein by double affinity electrophoresis with concanavalin A and Lens culinaris hemagglutinin A.
Electrophoresis '83(Ed. Hirai, H. )Berlin-New York. Walter de Gruyter & Co.   1984   
Regulation mechanisms of the human α-fetoprotein gene.
1992   
Human α-fetoprotein : Molecular biology of regulation mechanisms.
Schering-Plough Liver Forum(Ed. Y. Endo)Life Science Publicaing   1994   
Tissue culture of human hepatic cellular carcinoma
Satoi jiro, NAKABAYASHI Hidekazu (Part:Joint Work)
1982   
The primary culture and maintenance of normal and malignant liver cells.
Sato, J., Tokiwa, T,Nakabayashi, H., Miyazaki, M., and Miyano, K.
1980   

Conference Activities & Talks

 
TGF-β signaling cooperate with ATBF1 in AFP transcription.
Sakata, N., Kaneko, S., Ikeno, S., Miura, Y., Nakabayashi, H., Dong, XY., Dong, JT., Tamaoki, T., Nakano, N., Itoh, S.
The 3rd International Symposium by JSPS Core-to-Core Program “Cooperative International Framework in TGF-β Family Signaling”(Matsuyama)   2013   
TGF-β Signaling Cooperates with AT Motif Binding Factor 1 (ATBF1) to Repress α-Fetoprotein Gene in Human Hepatoma Cells.
2013   
The changes of Cuclopedic Glycan Patterns during the Process of Carcinogenesis.
Hiroko Fujita, Tomohisa Ogawa, Shunsaku Takeishi, Masao Yamada, Hidekazu Nakabayashi
Annual Conference of the Society for Glycobiology (Fort Worth, TX)   2008   
It is well known that glycoproteins are potentially useful tumor makers used in clinical applications. In determining the changes of the glycan patterns during the process of carcinogenesis, the safety is important for cultured embryonic stem cell...
Positive and negative regulation of the human α-fetoprotein gene
2003   
α-fetprotein(AFP) is abundantly expressed in liver during embryonic development. In adults, the protein is almost undetectable except in HCC patients. To examine tissue- and stage-specific expression of the gene, we found a potent liver-specific e...
Glucucorticoids stimulate the human but inhibit the rodent α-fetoprotein gene promoter
Nakabayahi, H., Koyama, Y., Sakai, M., Nishi, S.
27th Annual Meeting of ISOBM (Kyoto)   1999   
Glucocorticoids inhibit rodent α-fetoprotein(AFP) gene activity but stimulate expression of the human homologue. Like human, activity of the AFP promoter from other primates was stimulated by the synthetic glucocorticoid dexamethasone(Dex) in vari...
Altered lectin reactivities of α-fetoprotein and other glycoprotein in spent culture media of hepatoma cell lines
Taketa, K., Fukazawa, M.,, Nakabayashi, H., and Nishi, S.
26th Annual Meeting of ISOBM Umea, (Sweden)   1998   
α-Fetoprotein glycoforms of liver cancer at tissue and cell leveles
Taketa, K., Wang, S., Kamakura, K., Tanabe, M., Satoh, M., Ikeda, S., Tsuge, H., Akagi, T., Higashi, T., Tsuji, T., Nakabayashi, H., and Taga, H.
ISOBM-96 (San Diego)   1996   
Gene therapy : Application to the treatment of hepatocellular carcinoma
Hidekazu Nakabayashi, Hiroshi Suzuki, Taiki Tamaoki
31st Annual Meeting of Japan Society of Hepatology   1995   
Bovine serum-dependent alteration of lectin-reactive patterns of α-fetoprotein produced by HUH-6 cl-5 cells
Taketa, K., Nakabayashi, H., and Sato, J.
1987   
Micro heterogeneity of human α-fetoprotein produced by cultured cell lines of hepatocellular carcinoma
Taketa, K., Nakabayashi, H., Tokiwa, T., and Sato, J.
日本癌学会、第44回総会 (Tokyo).   1985   
In vitro thermo chemotherapy of human osteosarcoma cells with cis-dichlorodiammineplatium (II)
Yamane, Y., Nakabayashi, H., and Sumii, H.
1985   
Affinities to lectins of -fetoprotein and -Glutamyltransferase produced by cultured cell lines of human hepatocellular carcinoma and hepatoblastoma
NAKABAYASHI Hidekazu
Taketa, K., Izumi, M., Nakabayashi, H., Sato, J.   1983   
γ-Glutamyltransferase isozymes in the medium of cultured human hepatoma cell lines
Izumi, M., Taketa, K., Nakabayashi, H., and Sato, J.
The 40th Japanese Cancer Res. (Sapporo)   1981   

Research Grants & Projects

 
Molecular biology of gene expression mechanism of α-fetoprotein.
Gene therapeuitic approrch for the treatment of human hepatocellular corcinoma.

Patents

 
特許US Patent: 5804407, 5807738, 5827686, and 5843776. : Method of expressing genes in mammalian cells.
Tamaoki, T. and Nakabayashi, H.
The present invention relatest to a method of expressing a heterologous gene in mamalian cells and a recombinant DNA construct for use in the method. The invention also relates to a method of specifically killing cells which constitutively express...