2010年12月
Semicomprehensive Analysis of the Postnatal Age-Related Changes in the mRNA Expression of Sex Steroidogenic Enzymes and Sex Steroid Receptors in the Male Rat Hippocampus
ENDOCRINOLOGY
- ,
- ,
- ,
- ,
- 巻
- 151
- 号
- 12
- 開始ページ
- 5795
- 終了ページ
- 5806
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1210/en.2010-0581
- 出版者・発行元
- ENDOCRINE SOC
Although sex steroids play a crucial role in the postnatal brain development, the age-related changes in the hippocampal steroidogenesis remain largely unknown. We performed comprehensive investigations for the mRNA expressions of 26 sex steroidogenic enzymes/proteins and three sex steroid receptors in the male rat hippocampus, at the ages of postnatal day (PD) 1, PD4, PD7, PD10, PD14, 4 wk, and 12 wk (adult), by RT-PCR/Southern blotting analysis. The relative expression levels of these enzymes/receptors at PD1 were Srd5a1 > Star > Ar similar to Hsd17b4 similar to Hsd17b1 similar to Hsd17b7 similar to Esr1 similar to Srd5a2 > Hsd17b3 > Esr2 > Cyp11a1 > Cyp17a1 > Cyp19a1 similar to Hsd17b2 > 3 beta-hydroxysteroid dehydrogenase I. The mRNA levels of essential enzymes for progesterone/testosterone/estradiol metabolisms (Cyp17a1, Hsd17b7, and Cyp19a1) were approximately constant between PD1 and PD14 and then declined toward the adult levels. Cyp11a1 increased during PD4-PD14 and then considerably decreased toward the adult level (similar to 8% of PD1). Hsd17b1, Hsd17b2, and 3 beta-hydroxysteroid dehydrogenase I mRNA decreased approximately monotonously. Hsd17b3 increased to approximately 200% of PD1 during PD4-PD14 and was maintained at this high level. The 5 alpha-reductase mRNA was maintained constant (Srd5a1) or decreased monotonically (Srd5a2) toward the adult level. The Esr1 level peaked at PD4 and decreased toward the adult level, whereas Ar greatly increased during PD1-PD14 and was maintained at this high level. The Star and Hsd17b4 levels were maintained constant from neonate to adult. These results suggest that the hippocampal sex steroidogenic properties are substantially altered during the postnatal development processes, which might contribute to brain sexual maturation. (Endocrinology 151: 5795-5806, 2010)
- リンク情報
- ID情報
-
- DOI : 10.1210/en.2010-0581
- ISSN : 0013-7227
- Web of Science ID : WOS:000284489800024