2021年10月
VARIABILIDAD GENÉTICA EN Sechium spp. (Cucurbitaceae) EVALUADA CON MARCADORES AFLP
Agrociencia
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- 巻
- 55
- 号
- 7
- 開始ページ
- 611
- 終了ページ
- 626
- 記述言語
- スペイン語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.47163/agrociencia.v55i7.2607
- 出版者・発行元
- COLPOS
Using the Amplified Fragment Length Polymorphism (AFLP) technique, the genetic variability of a sample of 95 accessions of three species of Sechium (S. edule, S. chinantlense, S. compositum) was evaluated, with leaf DNA from the Banco Nacional de Germoplasma de Sechium edule en Mexico. Four combinations of AFPL were applied (EcoRI + ACC/MseI + CAC, EcoRI + ACC/MseI + CAT, EcoRI + ACC/MseI + CGC, and EcoRI + ACC/MseI + CGG). DNA samples were classified into three groups based on the flavour of the fruit (sweet, neutral, bitter). An average of 47.91% polymorphism, 0.16 heterozygosity, 32.83 number of polymorphic bands, and a zero Wright fixation index (Fst) was obtained. The evidence showed that the domesticated accessions (sweet, neutral) were separated from the bitter-taste genotypes. A monophyletic tree was generated with the genetic distance matrix and the neighbour-joining methodology. Analyses showed S. edule as the root taxon, deriving S. compositum and S. chinantlense as subgroups, and suggesting that there is not enough differentiation to treat them as separate species. The evaluated ample showed that there is no apparent reproductive barrier for genetic cross breeding. Genotypes behaved as a complex with evolutive dynamism; that genetic complexity would allow the design of new variants.
- ID情報
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- DOI : 10.47163/agrociencia.v55i7.2607
- ISSN : 1405-3195