2007年9月
Sensitive test for screening for Staphylococcus aureus in bovine mastitis by broth cultivation and PCR
VETERINARY RECORD
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- 巻
- 161
- 号
- 11
- 開始ページ
- 381
- 終了ページ
- 383
- 記述言語
- 英語
- 掲載種別
- 出版者・発行元
- BRITISH VETERINARY ASSOC
An assay was developed and evaluated for screening for Staphylococcus aureus in milk samples from cases of bovine mastitis by overnight cultivation in a broth containing 7-5 per cent sodium chloride, followed by PCR to amplify the nuc gene. The assay could detect concentrations of S aureus as low as 1 colony-forming unit/ml milk. Among 106 milk samples collected from individual quarters of lactating cows in one dairy herd and from a bulk tank, S aureus was detected in nine samples by the PCR assay but in only three samples by conventional microbiological culture.
- リンク情報
- ID情報
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- ISSN : 0042-4900
- Web of Science ID : WOS:000249901800010