2001年5月
Depth of invasion parallels increased cyclooxygenase-2 levels in patients with gastric carcinoma
CANCER
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- 巻
- 91
- 号
- 10
- 開始ページ
- 1876
- 終了ページ
- 1881
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1002/1097-0142(20010515)91:10<1876::AID-CNCR1209>3.0.CO;2-H
- 出版者・発行元
- JOHN WILEY & SONS INC
BACKGROUND. Nonsteroidal anti-inflammatory drugs may reduce the incidence of intestinal carcinoma, presumably through inhibition of cyclooxygenase-2 (COX-2). The authors correlated tumor expression of COX-2 with clinicopathologic features in tissues from patients with gastric carcinoma.
METHODS. Thirty-three surgical specimens, including carcinomas and corresponding noncancerous mucosa, were sampled. Reverse transcription-polymerase chain reaction analysis was performed concomitantly for COS-1 and COX-2. A COS-2 index was determined from the band density ratio of COX-2 to constitutively expressed COX-1. Immunohistochemical; staining with COX-2 antibody and routine histologic assessment were performed in the same specimens.
RESULTS. The COX-2 index in gastric carcinoma was significantly higher than in normal mucosa (3.4 +/- 0.7 vs. 2.2 +/- 0.7; P < 0.05). COS-2 indices were significantly higher in gastric carcinoma tissues with deep invasion; indices for pT1, pT2, pT3, and pT4 carcinomas were 0.8 <plus/minus> 0.31 2.8 +/- 0.5, 4.3 +/- 1.0, and 8.8 +/- 5.5, respectively (P < 0.05). Immunohistochemistry demonstrated COS-2 protein diffusely in the cytoplasm of tumor cells hut not in surrounding stroma or in noncancerous mucosa.
CONCLUSIONS, COX-2 mRNA expression in gastric carcinoma tissue is correlated closely with depth of invasion, indicating that COX-2 is involved in the growth of gastric carcinoma. <(c)> 2001 American Cancer Society.
METHODS. Thirty-three surgical specimens, including carcinomas and corresponding noncancerous mucosa, were sampled. Reverse transcription-polymerase chain reaction analysis was performed concomitantly for COS-1 and COX-2. A COS-2 index was determined from the band density ratio of COX-2 to constitutively expressed COX-1. Immunohistochemical; staining with COX-2 antibody and routine histologic assessment were performed in the same specimens.
RESULTS. The COX-2 index in gastric carcinoma was significantly higher than in normal mucosa (3.4 +/- 0.7 vs. 2.2 +/- 0.7; P < 0.05). COS-2 indices were significantly higher in gastric carcinoma tissues with deep invasion; indices for pT1, pT2, pT3, and pT4 carcinomas were 0.8 <plus/minus> 0.31 2.8 +/- 0.5, 4.3 +/- 1.0, and 8.8 +/- 5.5, respectively (P < 0.05). Immunohistochemistry demonstrated COS-2 protein diffusely in the cytoplasm of tumor cells hut not in surrounding stroma or in noncancerous mucosa.
CONCLUSIONS, COX-2 mRNA expression in gastric carcinoma tissue is correlated closely with depth of invasion, indicating that COX-2 is involved in the growth of gastric carcinoma. <(c)> 2001 American Cancer Society.
- リンク情報
- ID情報
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- DOI : 10.1002/1097-0142(20010515)91:10<1876::AID-CNCR1209>3.0.CO;2-H
- ISSN : 0008-543X
- Web of Science ID : WOS:000168497900005