SAWASAKI Tatsuya

J-GLOBAL         Last updated: Oct 2, 2019 at 09:36
 
Avatar
Name
SAWASAKI Tatsuya
Affiliation
Ehime University
Section
Proteo-Science Center Division of Ceii-Free Sciences
Job title
Professor
Degree
Ph. D.(Hiroshima University)

Research Areas

 
 

Academic & Professional Experience

 
Apr 2013
 - 
Today
Professor, Proteo-Science Center, Ehime University
 
Apr 2012
 - 
Mar 2013
Professor, Cell-free Science and Technology Center, Ehime University
 
Apr 2003
 - 
Mar 2012
Associate Professor, Cell-free Science and Technology Center, Ehime University
 
Apr 1999
 - 
Mar 2003
Assistant Professor, Applied Chemistry, Dept. Engineering, Ehime University
 
Oct 1998
 - 
Mar 1999
Postdoctoral Fellow, Applied Chemistry, Dept. Engineering, Ehime University
 

Education

 
 
 - 
1998
Graduate School, Division of Natural Science, Hiroshima University
 
 
   
 
Faculty of Science, Hiroshima University
 

Published Papers

 
Ali MRM, Uemura T, Ramadan A, Adachi K, Nemoto K, Nozawa A, Hoshino R, Abe H, Sawasaki T, Arimura GI
Plant physiology   179(4) 1273-1284   Apr 2019   [Refereed]
Maekawa M, Hiyoshi H, Nakayama J, Kido K, Sawasaki T, Semba K, Kubota E, Joh T, Higashiyama S
Biochemical and biophysical research communications   516(4) 1116-1122   Sep 2019   [Refereed]
Uematsu A, Kido K, Takahashi H, Takahashi C, Yanagihara Y, Saeki N, Yoshida S, Maekawa M, Honda M, Kai T, Shimizu K, Higashiyama S, Imai Y, Tokunaga F, Sawasaki T
The Journal of biological chemistry   294(38) 14135-14148   Sep 2019   [Refereed]
Miyamoto T, Uemura T, Nemoto K, Daito M, Nozawa A, Sawasaki T, Arimura GI
Frontiers in plant science   10 776   2019   [Refereed]
Miura T, Takeo S, Ntege EH, Otsuki H, Sawasaki T, Ishino T, Takashima E, Tsuboi T
Biochemical and biophysical research communications   500(2) 261-267   Jun 2018   [Refereed]
Hashimoto Y, Zhou W, Hamauchi K, Shirakura K, Doi T, Yagi K, Sawasaki T, Okada Y, Kondoh M, Takeda H
Scientific reports   8(1) 8383   May 2018   [Refereed]
Miyakawa K, Matsunaga S, Yokoyama M, Nomaguchi M, Kimura Y, Nishi M, Kimura H, Sato H, Hirano H, Tamura T, Akari H, Miura T, Adachi A, Sawasaki T, Yamamoto N, Ryo A
Nature communications   10(1) 1844   Apr 2019   [Refereed]
Nemoto K, Kagawa M, Nozawa A, Hasegawa Y, Hayashi M, Imai K, Tomii K, Sawasaki T
Scientific reports   8(1) 4268   Mar 2018   [Refereed]
Yonezawa T, Takahashi H, Shikata S, Sawasaki T, Kitamura T, Goyama S
Biochemical and biophysical research communications   505(3) 905-909   Nov 2018   [Refereed]
Uematsu A, Kido K, Manabe E, Takeda H, Takahashi H, Hayashi M, Imai Y, Sawasaki T
Biochemical and biophysical research communications   495(3) 2289-2295   Jan 2018   [Refereed]
Nakanishi A, Kaneko N, Takeda H, Sawasaki T, Morikawa S, Zhou W, Kurata M, Yamamoto T, Akbar SMF, Zako T, Masumoto J
Inflammation and regeneration   38 27   2018   [Refereed]
Hashimoto Y, Shirakura K, Okada Y, Takeda H, Endo K, Tamura M, Watari A, Sadamura Y, Sawasaki T, Doi T, Yagi K, Kondoh M
The Journal of pharmacology and experimental therapeutics   363(2) 275-283   Nov 2017   [Refereed]
Sun S, Nakashima K, Ito M, Li Y, Chida T, Takahashi H, Watashi K, Sawasaki T, Wakita T, Suzuki T
Scientific reports   7(1) 12874   Oct 2017   [Refereed]
Nemoto K, Ramadan A, Arimura GI, Imai K, Tomii K, Shinozaki K, Sawasaki T
Nature communications   8(1) 1004   Oct 2017   [Refereed]
[AGIA tag system: a super-sensitive detection and capture peptide tag suitable for cell biology].
Takeda H, Sawasaki T
Seikagaku. The Journal of Japanese Biochemical Society   89(2) 302-307   Apr 2017   [Refereed]
Liu S, Hasegawa H, Takemasa E, Suzuki Y, Oka K, Kiyoi T, Takeda H, Ogasawara T, Sawasaki T, Yasukawa M, Maeyama K
Journal of immunology (Baltimore, Md. : 1950)   199(5) 1584-1595   Sep 2017   [Refereed]
Kaneko Naoe, Ito Yuki, Iwasaki Tomoyuki, Takeda Hiroyuki, Sawasaki Tatsuya, Migita Kiyoshi, Agematsu Kazunaga, Koga Tomohiro, Kawakami Atsushi, Yachie Akihiro, Yoshiura Koh-ichiro, Morikawa Shinnosuke, Kurata Mie, Masumoto Junya
EUROPEAN JOURNAL OF INFLAMMATION   15(2) 85-97   Aug 2017   [Refereed]
Krug SM, Hayaishi T, Iguchi D, Watari A, Takahashi A, Fromm M, Nagahama M, Takeda H, Okada Y, Sawasaki T, Doi T, Yagi K, Kondoh M
Journal of controlled release : official journal of the Controlled Release Society   260 1-11   Aug 2017   [Refereed]
Yonezawa T, Takahashi H, Shikata S, Liu X, Tamura M, Asada S, Fukushima T, Fukuyama T, Tanaka Y, Sawasaki T, Kitamura T, Goyama S
The Journal of biological chemistry   292(30) 12528-12541   Jul 2017   [Refereed]
Yamamoto T, Taira Nihira N, Yogosawa S, Aoki K, Takeda H, Sawasaki T, Yoshida K
FEBS letters   591(6) 842-853   Mar 2017   [Refereed]
Ogawa S, Miyamoto K, Nemoto K, Sawasaki T, Yamane H, Nojiri H, Okada K
Scientific reports   7 40175   Jan 2017   [Refereed]
Shibata Y, Tokunaga F, Goto E, Komatsu G, Gohda J, Saeki Y, Tanaka K, Takahashi H, Sawasaki T, Inoue S, Oshiumi H, Seya T, Nakano H, Tanaka Y, Iwai K, Inoue JI
PLoS pathogens   13(1) e1006162   Jan 2017   [Refereed]
Takeda H, Zhou W, Kido K, Suno R, Iwasaki T, Kobayashi T, Sawasaki T
PloS one   12(5) e0178246   2017   [Refereed]
Nozawa A, Nemoto K, Nomura S, Yamanaka S, Kido K, Sawasaki T
Methods in molecular biology (Clifton, N.J.)   1621 37-46   2017   [Refereed]
Nemoto K, Nozawa A, Yamanaka S, Nomura S, Kido K, Sawasaki T
Methods in molecular biology (Clifton, N.J.)   1621 113-120   2017   [Refereed]
Kaneko N, Iwasaki T, Ito Y, Takeda H, Sawasaki T, Morikawa S, Nakano N, Kurata M, Masumoto J
Inflammation and regeneration   37 9   2017   [Refereed]
Sakamaki K, Ishii TM, Sakata T, Takemoto K, Takagi C, Takeuchi A, Morishita R, Takahashi H, Nozawa A, Shinoda H, Chiba K, Sugimoto H, Saito A, Tamate S, Satou Y, Jung SK, Matsuoka S, Koyamada K, Sawasaki T, Nagai T, Ueno N
Biochimica et biophysica acta   1863(11) 2766-2783   Nov 2016   [Refereed]
Takagi M, Sakamoto T, Suzuki R, Nemoto K, Obayashi T, Hirakawa T, Matsunaga TM, Kurihara D, Nariai Y, Urano T, Sawasaki T, Matsunaga S
Journal of plant research   129(6) 1165-1178   Nov 2016   [Refereed]
Kuwahara M, Ise W, Ochi M, Suzuki J, Kometani K, Maruyama S, Izumoto M, Matsumoto A, Takemori N, Takemori A, Shinoda K, Nakayama T, Ohara O, Yasukawa M, Sawasaki T, Kurosaki T, Yamashita M
Nature communications   7 12596   Sep 2016   [Refereed]
Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, Ishitani R, Kamei K, Takeyoshi I, Kawakami H, Iwai K, Hatada I, Sawasaki T, Ito H, Nureki O, Tokunaga F
Nature communications   7 12547   Aug 2016   [Refereed]
Santolini M, Sakakibara I, Gauthier M, Ribas-Aulinas F, Takahashi H, Sawasaki T, Mouly V, Concordet JP, Defossez PA, Hakim V, Maire P
Nucleic acids research      Jun 2016   [Refereed]
Suzuki J, Maruyama S, Tamauchi H, Kuwahara M, Horiuchi M, Mizuki M, Ochi M, Sawasaki T, Zhu J, Yasukawa M, Yamashita M
Immunology   147(4) 476-487   Apr 2016   [Refereed]
Shi G, Ando T, Suzuki R, Matsuda M, Nakashima K, Ito M, Omatsu T, Oba M, Ochiai H, Kato T, Mizutani T, Sawasaki T, Wakita T, Suzuki T
PLoS pathogens   12(2) e1005441   Feb 2016   [Refereed]
Suzuki Y, Chin WX, Han Q, Ichiyama K, Lee CH, Eyo ZW, Ebina H, Takahashi H, Takahashi C, Tan BH, Hishiki T, Ohba K, Matsuyama T, Koyanagi Y, Tan YJ, Sawasaki T, Chu JJ, Vasudevan SG, Sano K, Yamamoto N
PLoS pathogens   12(1) e1005357   Jan 2016   [Refereed]
Kudoh A, Miyakawa K, Matsunaga S, Matsushima Y, Kosugi I, Kimura H, Hayakawa S, Sawasaki T, Ryo A
Frontiers in microbiology   7 883   2016   [Refereed]
Iwasaki T, Kaneko N, Ito Y, Takeda H, Sawasaki T, Heike T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J
TheScientificWorldJournal   2016 2597376   2016   [Refereed]
Takahashi H, Uematsu A, Yamanaka S, Imamura M, Nakajima T, Doi K, Yasuoka S, Takahashi C, Takeda H, Sawasaki T
PloS one   11(6) e0156718   2016   [Refereed]
Yano T, Takeda H, Uematsu A, Yamanaka S, Nomura S, Nemoto K, Iwasaki T, Takahashi H, Sawasaki T
PloS one   11(6) e0156716   2016   [Refereed]
Kaneko N, Ito Y, Iwasaki T, Takeda H, Sawasaki T, Migita K, Agematsu K, Kawakami A, Morikawa S, Mokuda S, Kurata M, Masumoto J
Journal of immunological methods   426 76-81   Nov 2015   [Refereed]
Ramadan A, Nemoto K, Seki M, Shinozaki K, Takeda H, Takahashi H, Sawasaki T
BMC plant biology   15 275   Nov 2015   [Refereed]
Hirotaka Takahashi, Keiichirou Nemoto, Ramadan Abdelaziz, Atsushi Uematsu, Tatsuya Sawasaki
Protein Modifications in Pathogenic Dysregulation of Signaling   43-60   Sep 2015   [Refereed]
© Springer Japan 2015. All rights are reserved. Post-translational modifications have crucial roles in the regulation of many physiological processes such as development, differentiation, and response to extracellular signals. Currently, protein p...
Nakajima M, Nagase S, Iida M, Takeda S, Yamashita M, Watari A, Shirasago Y, Fukasawa M, Takeda H, Sawasaki T, Yagi K, Kondoh M
The Journal of pharmacology and experimental therapeutics   354(3) 440-447   Sep 2015   [Refereed]
Ohnishi T, Yanazawa M, Sasahara T, Kitamura Y, Hiroaki H, Fukazawa Y, Kii I, Nishiyama T, Kakita A, Takeda H, Takeuchi A, Arai Y, Ito A, Komura H, Hirao H, Satomura K, Inoue M, Muramatsu S, Matsui K, Tada M, Sato M, Saijo E, Shigemitsu Y, Sakai S, Umetsu Y, Goda N, Takino N, Takahashi H, Hagiwara M, Sawasaki T, Iwasaki G, Nakamura Y, Nabeshima Y, Teplow DB, Hoshi M
Proceedings of the National Academy of Sciences of the United States of America   112(32) E4465-74   Aug 2015   [Refereed]
Nemoto K, Takemori N, Seki M, Shinozaki K, Sawasaki T
The Journal of biological chemistry   290(27) 16665-16677   Jul 2015   [Refereed]
Takeda H, Ogasawara T, Ozawa T, Muraguchi A, Jih PJ, Morishita R, Uchigashima M, Watanabe M, Fujimoto T, Iwasaki T, Endo Y, Sawasaki T
Scientific reports   5 11333   Jun 2015   [Refereed]
Takemori N, Takemori A, Matsuoka K, Morishita R, Matsushita N, Aoshima M, Takeda H, Sawasaki T, Endo Y, Higashiyama S
Molecular bioSystems   11(2) 361-365   Feb 2015   [Refereed]
Sharmy J. James, Sharmy J. James, Huipeng Jiao, Huipeng Jiao, Hong Ying Teh, Hong Ying Teh, Hirotaka Takahashi, Hirotaka Takahashi, Chin Wen Png, Chin Wen Png, Meng Chee Phoon, Youichi Suzuki, Tatsuy Sawasaki, Hui Xiao, Vincent T.K. Chow, Naoki Yamamoto, Joseph M. Reynolds, Richard A. Flavell, Chen Dong, Yongliang Zhang, Yongliang Zhang
Cell Reports   10 1722-1734   Jan 2015
© 2015 The Authors. The type I interferon system is essential for antiviral immune response and is a primary target of viral immune evasion strategies. Here, we show that virus infection induces the expression of MAPK phosphatase 5 (MKP5), a dual-...
Onishi S, Adnan E, Ishizaki J, Miyazaki T, Tanaka Y, Matsumoto T, Suemori K, Shudou M, Okura T, Takeda H, Sawasaki T, Yasukawa M, Hasegawa H
PloS one   10(6) e0126564   2015   [Refereed]
Kinoshita-Kikuta E, Kinoshita E, Eguchi Y, Yanagihara S, Edahiro K, Inoue Y, Taniguchi M, Yoshida M, Yamamoto K, Takahashi H, Sawasaki T, Utsumi R, Koike T
PloS one   10(7) e0132598   2015   [Refereed]
Furukawa H, Oka S, Shimada K, Masuo K, Nakajima F, Funano S, Tanaka Y, Komiya A, Fukui N, Sawasaki T, Tadokoro K, Nose M, Tsuchiya N, Tohma S
Biomarker insights   10 63-73   2015   [Refereed]
Matsunaga S, Masaoka T, Sawasaki T, Morishita R, Iwatani Y, Tatsumi M, Endo Y, Yamamoto N, Sugiura W, Ryo A
Frontiers in microbiology   6 1220   2015   [Refereed]
Sakamaki K, Shimizu K, Iwata H, Imai K, Satou Y, Funayama N, Nozaki M, Yajima M, Nishimura O, Higuchi M, Chiba K, Yoshimoto M, Kimura H, Gracey AY, Shimizu T, Tomii K, Gotoh O, Akasaka K, Sawasaki T, Miller DJ
Molecular biology and evolution   31(12) 3282-3301   Dec 2014   [Refereed]
Arimitsu E, Ogasawara T, Takeda H, Sawasaki T, Ikeda Y, Hiasa Y, Maeyama K
European journal of pharmacology   745 117-122   Dec 2014   [Refereed]
Fu SC, Imai K, Sawasaki T, Tomii K
Proteomics   14(17-18) 2042-2046   Sep 2014   [Refereed]
Tan BH, Suzuki Y, Takahashi H, Ying PH, Takahashi C, Han Q, Chin WX, Chao SH, Sawasaki T, Yamamoto N, Suzuki Y
The Journal of biological chemistry   289(38) 26368-26382   Sep 2014   [Refereed]
Shimizu K, Uematsu A, Imai Y, Sawasaki T
FEBS letters   588(17) 3030-3037   Aug 2014   [Refereed]
Masaki T, Matsunaga S, Takahashi H, Nakashima K, Kimura Y, Ito M, Matsuda M, Murayama A, Kato T, Hirano H, Endo Y, Lemon SM, Wakita T, Sawasaki T, Suzuki T
Journal of virology   88(13) 7541-7555   Jul 2014   [Refereed]
Takiuchi T, Nakagawa T, Tamiya H, Fujita H, Sasaki Y, Saeki Y, Takeda H, Sawasaki T, Buchberger A, Kimura T, Iwai K
Genes to cells : devoted to molecular & cellular mechanisms   19(3) 254-272   Mar 2014   [Refereed]
Y. Mizutani, S. Tsuge, S. Tsuge, H. Takeda, Y. Hasegawa, K. Shiogama, T. Onouchi, K. Inada, T. Sawasaki, Y. Tsutsumi
Molecular Oral Microbiology   29 156-173   Jan 2014
Porphyromonas gingivalis is a keystone periodontal pathogen. Histologocally, the gingival tissue in periodontitis shows dense infiltration of plasma cells. However, antigens recognized by antibodies secreted from the immunocytes remain unknown. Th...
Kudoh A, Takahama S, Sawasaki T, Ode H, Yokoyama M, Okayama A, Ishikawa A, Miyakawa K, Matsunaga S, Kimura H, Sugiura W, Sato H, Hirano H, Ohno S, Yamamoto N, Ryo A
Retrovirology   11(1) 9-9   Jan 2014   [Refereed]
Mitsunami T, Nishihara M, Galis I, Alamgir KM, Hojo Y, Fujita K, Sasaki N, Nemoto K, Sawasaki T, Arimura G
PloS one   9(9) e108849-e108849   2014   [Refereed]
Ishigami T, Abe K, Aoki I, Minegishi S, Ryo A, Matsunaga S, Matsuoka K, Takeda H, Sawasaki T, Umemura S, Endo Y
FASEB journal : official publication of the Federation of American Societies for Experimental Biology   27(9) 3437-3445   Sep 2013   [Refereed]
Germain H, Gray-Mitsumune M, Houde J, Benhamman R, Sawasaki T, Endo Y, Matton DP
Plant physiology and biochemistry : PPB / Societe francaise de physiologie vegetale   70 261-268   Sep 2013   [Refereed]
Shimizu K, Sawasaki T
FEBS letters   587(14) 2219-2225   Jul 2013   [Refereed]
[Cell-free based protein array technology for analyses of protein kinases and ubiquitin ligases].
Sawasaki T, Takeda H, Takahashi H, Nemoto K
Seikagaku. The Journal of Japanese Biochemical Society   85(6) 438-446   Jun 2013   [Refereed]
Iwasaki T, Katayama T, Kohama K, Endo Y, Sawasaki T
Molecular biology of the cell   24(6) 748-756   Mar 2013   [Refereed]
Mizutani Y, Matsuoka K, Takeda H, Shiogama K, Inada K, Hayakawa K, Yamada H, Miyazaki T, Sawasaki T, Endo Y, Tsutsumi Y
Journal of immunological methods   387(1-2) 57-70   Jan 2013   [Refereed]
Nakano M, Nishihara M, Yoshioka H, Takahashi H, Sawasaki T, Ohnishi K, Hikichi Y, Kiba A
PloS one   8(9) e75124   2013   [Refereed]
Takahashi H, Takahashi C, Moreland NJ, Chang YT, Sawasaki T, Ryo A, Vasudevan SG, Suzuki Y, Yamamoto N
Antiviral research   96(3) 305-314   Dec 2012   [Refereed]
Kominami K, Nakabayashi J, Nagai T, Tsujimura Y, Chiba K, Kimura H, Miyawaki A, Sawasaki T, Yokota H, Manabe N, Sakamaki K
Biochimica et biophysica acta   1823(10) 1825-1840   Oct 2012   [Refereed]
Miyakawa K, Sawasaki T, Matsunaga S, Tokarev A, Quinn G, Kimura H, Nomaguchi M, Adachi A, Yamamoto N, Guatelli J, Ryo A
Science signaling   5(245) ra73   Oct 2012   [Refereed]
Takahashi H, Ozawa A, Nemoto K, Nozawa A, Seki M, Shinozaki K, Takeda H, Endo Y, Sawasaki T
FEBS letters   586(19) 3134-3141   Sep 2012   [Refereed]
Matsunaga S, Sawasaki T, Ode H, Morishita R, Furukawa A, Sakuma R, Sugiura W, Sato H, Katahira M, Takaori-Kondo A, Yamamoto N, Ryo A
Journal of proteomics   75(15) 4863-4873   Aug 2012   [Refereed]
Kiba A, Nakano M, Vincent-Pope P, Takahashi H, Sawasaki T, Endo Y, Ohnishi K, Yoshioka H, Hikichi Y
Journal of plant physiology   169(10) 1017-1022   Jul 2012   [Refereed]
Shimizu K, Takahama S, Endo Y, Sawasaki T
PloS one   7(8) e42721-e42721   2012   [Refereed]
Kominami K, Nagai T, Sawasaki T, Tsujimura Y, Yashima K, Sunaga Y, Tsuchimochi M, Nishimura J, Chiba K, Nakabayashi J, Koyamada K, Endo Y, Yokota H, Miyawaki A, Manabe N, Sakamaki K
PloS one   7(11) e50218   2012   [Refereed]
Nemoto K, Seto T, Takahashi H, Nozawa A, Seki M, Shinozaki K, Endo Y, Sawasaki T
Phytochemistry   72(10) 1136-1144   Jul 2011   [Refereed]
Tsuge S, Mizutani Y, Matsuoka K, Sawasaki T, Endo Y, Naruishi K, Maeda H, Takashiba S, Shiogama K, Inada K, Tsutsumi Y
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society   59(7) 673-689   Jul 2011   [Refereed]
Akagi T, Shimizu K, Takahama S, Iwasaki T, Sakamaki K, Endo Y, Sawasaki T
FEBS letters   585(12) 1835-1840   Jun 2011   [Refereed]
Madono M, Sawasaki T, Morishita R, Endo Y
New biotechnology   28(3) 211-217   Apr 2011   [Refereed]
Nozawa A, Ogasawara T, Matsunaga S, Iwasaki T, Sawasaki T, Endo Y
BMC biotechnology   11(1) 35-35   Apr 2011   [Refereed]
Shin ichi Makino, Shin ichi Makino, Shin ichi Makino, Tatsuya Sawasaki, Tatsuya Sawasaki, Tatsuya Sawasaki, Yaeta Endo, Yaeta Endo, Yaeta Endo, Kazuyuki Takai, Kazuyuki Takai, Kazuyuki Takai
Biochemical and Biophysical Research Communications   404(4) 1050-1054   Jan 2011   [Refereed]
Wheat RNA ligase can be dissected into three isolated domain enzymes that are responsible for its core ligase, 5'-kinase, and 2',3'-cyclic phosphate 3'-phosphodiesterase activities, respectively. In the present study, we pursued a practical strate...
Chidananda Nagamangala Kanchiswamy, Chidananda Nagamangala Kanchiswamy, Chidananda Nagamangala Kanchiswamy, Atsushi Muroi, Atsushi Muroi, Massimo E. Maffei, Hirofumi Yoshioka, Tatsuya Sawasaki, Gen Ichiro Arimura, Gen Ichiro Arimura
Plant Biotechnology   27(5) 469-473   Dec 2010   [Refereed]
Little is known about the mechanisms by which Ca2+-binding sensory proteins direct the plant heat shock (HS) response. Since two Ca2+-dependent protein kinases (CPK3 and CPK13) were recently shown to phosphorylate the heat shock transcription fact...
D. Tadokoro, S. Takahama, K. Shimizu, S. Hayashi, Y. Endo, Y. Endo, Y. Endo, T. Sawasaki, T. Sawasaki, T. Sawasaki
Cell Death and Disease   1(1) e89-e89   Oct 2010   [Refereed]
Caspase-3 (CASP3) cleaves many proteins including protein kinases (PKs). Understanding the relationship(s) between CASP3 and its PK substrates is necessary to delineate the apoptosis signaling cascades that are controlled by CASP3 activity. We rep...
Kazuhiro Matsuoka, Hiroaki Komori, Masato Nose, Masato Nose, Yaeta Endo, Yaeta Endo, Yaeta Endo, Yaeta Endo, Tatsuya Sawasaki, Tatsuya Sawasaki, Tatsuya Sawasaki, Tatsuya Sawasaki
Journal of Proteome Research   9(8) 4264–4273-4273   Aug 2010   [Refereed]
Autoimmune diseases are a heterogeneous group of diseases characterized by immune reactions against either a major or a limited number of the bodies own autoantigens, causing inflammation and damage to tissues and organs. Thus, identification of a...
Shin ichi Makino, Shin ichi Makino, Tatsuya Sawasaki, Tatsuya Sawasaki, Tatsuya Sawasaki, Yaeta Endo, Yaeta Endo, Yaeta Endo, Kazuyuki Takai, Kazuyuki Takai, Kazuyuki Takai
Biochemical and Biophysical Research Communications   397(4) 762-766   Jul 2010   [Refereed]
Wheat RNA ligase contains 5'-hydroxyl kinase, 2',3'-cyclic phosphate 3'-phosphodiesterase, and 5'-phosphate 2'-phosphate-3'-hydroxyl RNA ligase activities in a 110-kDa polypeptide. Taking advantage of a wheat cell-free protein production system, w...
Satoko Matsunaga, Kazuhiro Matsuoka, Kouhei Shimizu, Yaeta Endo, Yaeta Endo, Yaeta Endo, Yaeta Endo, Tatsuya Sawasaki, Tatsuya Sawasaki, Tatsuya Sawasaki, Tatsuya Sawasaki
BMC Biotechnology   10(1) 42-42   Jun 2010   [Refereed]
Background: Technology used for the purification of recombinant proteins is a key issue for the biochemical and structural analyses of proteins. In general, affinity tags, such as glutathione-S-transferase or six-histidines, are used to purify rec...
Chidananda N. Kanchiswamy, Chidananda N. Kanchiswamy, Chidananda N. Kanchiswamy, Hirotaka Takahashi, Hirotaka Takahashi, Stefano Quadro, Massimo E. Maffei, Simone Bossi, Cinzia Bertea, Simon A. Zebelo, Atsushi Muroi, Atsushi Muroi, Nobuaki Ishihama, Hirofumi Yoshioka, Wilhelm Boland, Junji Takabayashi, Yaeta Endo, Tatsuya Sawasaki, Gen Ichiro Arimura, Gen Ichiro Arimura
BMC Plant Biology   10(1) 97-97   May 2010   [Refereed]
Background: Plant Ca2+ signals are involved in a wide array of intracellular signaling pathways after pest invasion. Ca2+-binding sensory proteins such as Ca2+-dependent protein kinases (CPKs) have been predicted to mediate the signaling following...
Kazuyuki Takai, Tatsuya Sawasaki, Yaeta Endo
Current Pharmaceutical Biotechnology   11(3) 272-238   Apr 2010   [Refereed]
We have made a dramatic improvement of the wheat cell-free protein synthesis system. The first key improvement is the method for preparation of the cell-free extract that is free of inhibitory factors of translation reaction. Additional improvemen...
Yuki Tanaka, Yuki Tanaka, Hiroaki Komori, Shiro Mori, Yoshiko Soga, Takahito Tsubaki, Miho Terada, Tatsuhiko Miyazaki, Takahiro Fujino, Satoshi Nakamura, Hiroyuki Kanno, Tatsuya Sawasaki, Yaeta Endo, Masato Nose, Masato Nose
Tohoku Journal of Experimental Medicine   220(3) 199-206   Mar 2010   [Refereed]
Enzyme-linked immunosorbent assays (ELISA) have been widely used to determine quantitatively autoantibodies. However, the processes for the purification and immobilization of antigens in conventional ELISA methods include multiple steps, which hav...
Kazuyuki Takai, Tatsuya Sawasaki, Yaeta Endo
Nature Protocols   5(2) 227-238   Feb 2010   [Refereed]
Biochemical characterization of each gene product encoded in the genome is essential to understand how cells are regulated. The bottleneck has been and still is in how the gene products can be obtained. The wheat cell-free protein synthesis system...
Sawasaki T, Endo Y
Methods in molecular biology (Clifton, N.J.)   607 63-72   2010   [Refereed]
Tsuboi T, Takeo S, Sawasaki T, Torii M, Endo Y
Methods in molecular biology (Clifton, N.J.)   607 73-83   2010   [Refereed]
Watanabe M, Miyazono K, Tanokura M, Sawasaki T, Endo Y, Kobayashi I
Methods in molecular biology (Clifton, N.J.)   607 149-160   2010   [Refereed]
Hiroki Shimada, Kei Ichi Hirai, Eriko Simamura, Toshihisa Hatta, Hiroki Iwakiri, Keiji Mizuki, Taizo Hatta, Tatsuya Sawasaki, Tatsuya Sawasaki, Satoko Matsunaga, Yaeta Endo, Yaeta Endo, Shigeomi Shimizu
Journal of Biological Chemistry   284(42) 28642-28649   Oct 2009   [Refereed]
Paraquat (PQ), a herbicide used worldwide, causes fatal injury to organs upon high dose ingestion. Treatments for PQ poisoning areunreliable,andnumerousdeathshavebeenattributedinappropriate usage of the agent. It is generally speculated that a mic...
[Membrane protein production using wheat germ cell-free system].
Nozawa A, Tozawa Y, Sawasaki T, Endo Y
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   54(12 Suppl) 1443-1447   Sep 2009   [Refereed]
Akira Nozawa, Akira Nozawa, Yuko Matsubara, Yoshinori Tanaka, Hirotaka Takahashi, Tatsuya Akagi, Motoaki Seki, Kazuo Shinozaki, Yaeta Endo, Yaeta Endo, Tatsuya Sawasaki, Tatsuya Sawasaki
Bioscience, Biotechnology and Biochemistry   73(7) 1661-1664   Aug 2009   [Refereed]
We created a protein library consisting of 647 Arabidopsis transcription factors (TFs) using a wheat cell-free system. The quality of proteins in the library was checked by binding assay of bZIP family proteins. Screening of TFs binding to 5′-regu...
Hirotaka Takahashi, Hirotaka Takahashi, Akira Nozawa, Akira Nozawa, Akira Nozawa, Motoaki Seki, Kazuo Shinozaki, Kazuo Shinozaki, Kazuo Shinozaki, Kazuo Shinozaki, Yaeta Endo, Yaeta Endo, Yaeta Endo, Tatsuya Sawasaki, Tatsuya Sawasaki, Tatsuya Sawasaki
BMC Plant Biology   9(1) 39-39   May 2009   [Refereed]
Background. Ubiquitination is mediated by the sequential action of at least three enzymes: the E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme) and E3 (ubiquitin ligase) proteins. Polyubiquitination of target proteins is also im...
Nishi M, Ryo A, Tsurutani N, Ohba K, Sawasaki T, Morishita R, Perrem K, Aoki I, Morikawa Y, Yamamoto N
FEBS letters   583(8) 1243-1250   Apr 2009   [Refereed]

Misc

 
The transcriptional repressor Bach2 controls Th2-type immune response via interaction with Batf
Kuwahara Makoto, Sawasaki Tatsuya, Yamashita Masakatsu
CYTOKINE   100 34   Dec 2017   [Refereed]
Pharmacological analysis of Gs GTP-binding protein alpha subunit synthesized using a wheat germ cell-free protein synthesis system
Arimitsu Eiji, Ogasawara Tomio, Takeda Hiroyuki, Sawasaki Tatsuya, Ikeda Yoshio, Hiasa Yoichi, Suzuki Yasuyuki, Maeyama Kazutaka
JOURNAL OF PHARMACOLOGICAL SCIENCES   133(3) S109   Mar 2017   [Refereed]
The Efficiency of the Regulation of Ca2+Entry through Calcium Release-Activated Calcium Channel in the Treatment of Rheumatoid Arthritis
Liu Shuang, Hasegawa Hitoshi, Kiyoi Takeshi, Sawasaki Tatsuya, Maeyama Kazutaka
ARTHRITIS & RHEUMATOLOGY   68    Oct 2016   [Refereed]
Nagayoshi Y, Nakamura M, Matsuoka K, Ohtsuka T, Mori Y, Kono H, Aso T, Ideno N, Takahata S, Ryo A, Takeda H, Ito T, Oda Y, Endo Y, Sawasaki T, Tanaka M
Annals of surgical oncology   21 Suppl 3 S459-65   Jun 2014   [Refereed]
Ito M, Takahashi H, Sawasaki T, Ohnishi K, Hikichi Y, Kiba A
Plant signaling & behavior   9(1) e27420   2014   [Refereed]
Wheat cell-free technology for production and functional analysis of membrane protein
Sawasaki Tatsuya
JOURNAL OF PHARMACOLOGICAL SCIENCES   124 62P   2014   [Refereed]
Pharmacological analysis of membrane protein receptors synthesized using a wheat germ cell-free system with liposomes
Arimitsu Eiji, Ogasawara Tomio, Takeda Hiroyuki, Hiasa Yoich, Sawasaki Tatsuya, Maeyama Kazutakaa
JOURNAL OF PHARMACOLOGICAL SCIENCES   124 111P   2014   [Refereed]
Kazuyuki Takai, Tatsuya Sawasaki, Yaeta Endo
Plant Cell Monographs   18 27-39   Dec 2010
Mammalian and bacterial ribosomes have ribosomal RNAs comprising 7,000 and 5,000 nucleotides, respectively. The RNA N-glycosidase activity of ricin and other ribosome-inactivating proteins (RIPs) specifically catalyzes removal of single adenine in...
Pharmacological analysis of GPCRs and ion channels synthesized using a wheat germ cell-free system with liposomes
Maeyama Kazutaka, Liu Shuan, Arimitsu Eiji, Sawasaki Tatsuya
JOURNAL OF PHARMACOLOGICAL SCIENCES   124 62P   2014   [Refereed]
Hiroyuki Takeda, Tatsuya Sawasaki
Seikagaku   89 302-307   Jan 2017
RHOPH COMPLEX FROM MOUSE MALARIA PARASITE INTERACTS WITH ERYTHROCYTE CALMYRIN
Takeo Satoru, Miura Toyokazu, Otsuki Hitoshi, Sawasaki Tatsuya, Endo Yaeta, Torii Motomi, Tsuboi Takafumi
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE   83(5) 281-282   Nov 2010   [Refereed]
Gen Ichiro Arimura, Gen Ichiro Arimura, Tatsuya Sawasaki
Plant Signaling and Behavior   5(10) 1263-1265   Oct 2010
Plant Ca 2+-dependent protein kinase (CPK) signaling is involved in a wide array of intracellular signaling pathways involved in stomatal movement and plant adaptation to various environmental challenges including drought, salt and cold stress. Ar...
Tatsuya Sawasaki, Yaeta Endo
Cell-Free Protein Synthesis: Methods and Protocols   111-139   Jul 2008
Takafumi Tsuboi, Takafumi Tsuboi, Takafumi Tsuboi, Satoru Takeo, Hideyuki Iriko, Hideyuki Iriko, Ling Jin, Masateru Tsuchimochi, Shusaku Matsuda, Eun Taek Han, Eun Taek Han, Hitoshi Otsuki, Osamu Kaneko, Osamu Kaneko, Jetsumon Sattabongkot, Rachanee Udomsangpetch, Tatsuya Sawasaki, Motomi Torii, Yaeta Endo
Infection and Immunity   76(4) 1702-1708   Apr 2008
One of the major bottlenecks in malaria research has been the difficulty in recombinant protein expression. Here, we report the application of the wheat germ cell-free system for the successful production of malaria proteins. For proof of principl...
Discovering novel blood stage malaria vaccine candidates: Screening with immune sera from falciparum malaria patients and asymptomatic parasite carriers
Takeo Satoru, Jin Ling, Sakamoto Hirokazu, Han Eun-Taek, Iriko Hideyuki, Kaneko Osamu, Torii Motomi, Sattabongkot Jetsumon, Udomsangpetch Rachanee, Sawasaki Tatsuya, Endo Yaeta, Tsuboi Takafumi
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE   75(5) 302   Nov 2006   [Refereed]
High-throughput screening for asexual blood stage plasmodium falciparum vaccine candidates
Takeo Satoru, Iriko Hicleyuki, Jin Ling, Tsuchimochi Masateru, Han Eun-Taek, Kaneko Osamu, Torii Motomi, Sattabongkot Jetsumon, Udomsangpetch Rachanee, Sawasaki Tatsuya, Endo Yaeta, Tsuboi Takafumi
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE   73(6) 138   Dec 2005   [Refereed]
Yaeta Endo, Tomio Ogasawara, Tatsuya Sawasaki
Kinoshi Kenkyu Kaishi/Annals of the High Performance Paper Society, Japan   40 69-73   Dec 2001
Materialization of genetic information is an essential step toward modern biology in both basic and applied fields today. We reported a novel system for massive production of gene products, which is based on wheat germ cell-free translation system...
Transgenic "Air-pollutant-philic plants" produced by particle bombardment(共著)
Research in Photosynthesis, Klumer Academic Publishers.   IV 83-86   1992
Structures of transgcne loci in transgenic Arobidopsis plants obtaincd by porticle bombardment : Jvnction vegions can bind to nudear matrices
Gcne   218(1) 27-35   1998
Formation of circular polysomes in wheat germ cell-free protein synthesis system
Elsevier ScienceFEBS Letters   555(3) 455-458   2004
Elsevier B.V.New Biotechnology   in press    2010

Books etc

 
Chemical Genomics: Reviews and protocols. Methods in Molecular Biology
Humana Press   2005   
Methods in Molecular biology: In vitro transcription and translation
Humana Press   2007   
Cell-Free Translation Systems
Springer-Verlag   2002   
Method in Enzymolog
Academic Press   2001   
Chemical Genomics: Reviews and protocols. Methods in Molecular Biology
Humana Press   2005   
"Structural Genomics, Part A", Advances in Protein Chemistry and Structural Biology, Vol. 75
Elsevier Inc.   2008   
Expression Systems
Scion Publishing Ltd.   2007   
Cell-free Protein Synthesis
WILEY-VCH Verlag GmbH & Co. KGaA   2008   
Methods in Molecular Biology, vol607, Cell-Free Protein Production : Methods and Protocols
Humana Press   2009   
Cell-free protein production: Methods and protocols. Methods in Molecular Biology 607
Humana Press, a part of Springer Science+Business Media   2010   
Cell-free protein production: Methods and protocols. Methods in Molecular Biology 607
Humana Press, a part of Springer Science+Business Media   2010   
Cell-free protein production: Methods and protocols. Methods in Molecular Biology 607
Humana Press, a part of Springer Science+Business Media   2010   

Conference Activities & Talks

 
Establishment of robust assay platform based on cell-free protein array to analyze ubiquitin-mediated signal transductions
Hirotaka Takahashi, Atsushi Uematsu, Hiroyuki Takeda and Tatsuya Sawasaki
The Ubiquitin System: From Basic Science to Drug Discovery (A2)   8 Jan 2014   Keystone Sympodia
Identification of a Novel E3 Ligase Responsible for Ubiquitination and Subsequent Degradation of Tumor Suppressor Protein PTEN by Wheat Cell-free Based Protein Array
13 Sep 2013   
Masato Abe, Hiroyuki Hori, Takeshi Nakanishi, Fumio Arisaka, Tomio Ogasawara, Tatsuya Sawasaki, Masaya Kitamura, Yaeta Endo
Nucleic acids symposium series (2004)   1 Dec 2004   
To develop applications of in vitro cell-free translation systems for production and characterization of cofactor binding proteins, we investigate the production of apo- or holo-forms of Flavin Mono Nucleotide (FMN)-binding protein from Desulfovib...
T. Sawasaki, Y. Hasegawa, M. Tsuchimochi, Y. Kasahara, Y. Endo
Nucleic acids symposium series   1 Jan 2000   
Using the expression vector, pEU, which we have constructed, highly efficient in vitro protein synthesis can be achieved: The system works for 150 hours and without further template addition once the reaction has started, yielding 5 mg of enzymati...
T. Sawasaki, R. Morishita, A. Ozawa, T. Ogasawara, K. Madin, Y. Endo
Nucleic acids symposium series   1 Jan 1999   
A new enzyme, which we named ribosome RNA apurinic site specific lyase (RALyase), has been characterized. The enzyme specifically cleaves a phosphodiester bond at the apurinic site in the sarcin/ricin domain of 28S rRNA in ribosomes. The cut ends ...

Research Grants & Projects

 
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(B))
Project Year: 2010 - 2012    Investigator(s): Tatsuya SAWASAKI
Using a cell-free protein array constructed by a wheat cell-free protein synthesis system, comprehensive screening of caspase-3-cleaved protein kinases was carried out. Newly 30 protein kinases were found as substrates of caspase-3. Almost of clea...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(挑戦的萌芽研究)
Project Year: 2012 - 2013    Investigator(s): Koichiro OHMURA
Anti-cyclic citrullinated peptide (CCP) antibody is a specific autoantibody against rheumatoid arthritis (RA). However, it becomes positive in only about 50% of early RA patients, and sometimes it is difficult to correctly diagnose RA in the early...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2011 - 2013    Investigator(s): Shigeaki OHNO
In this study, serum autoantigens were examined comprhensively in the patients with endogenous uveitis by using our novel detection system.Serum samples were divided into 3 pooled groups.Among Vogt-Koyanagi-Harada disease patients, 10 kinds of pro...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(挑戦的萌芽研究)
Project Year: 2011 - 2012    Investigator(s): Nobuhisa MIZUKI
The aim of this study was to investigate risk factors associated with non-response to infliximab treatment for Behcet’s disease. We found some candidate proteins associated with non-response to infliximab treatment using wheat cell-free protein sy...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(挑戦的萌芽研究)
Project Year: 2010 - 2011    Investigator(s): Shigeki HIGASHIYAMA
HB-EGF and AREG are synthesized as a membrane-anchored growth factor and expressed at the plasma membrane as pro-forms in normal cells. In some malignant tumor cells in culture, however, proHB-EGF and proAREG trans-locate at the ER/nuclear membran...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(A))
Project Year: 2009 - 2011    Investigator(s): Takafumi TSUBOI
In order to identify novel molecules localized in the apical organelles of Plasmodium merozoite, we have expressed 193 putative merozoite specific molecules and produced antibodies against each recombinant protein. Among them, 29 were localized at...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(若手研究(B))
Project Year: 2009 - 2010    Investigator(s): Genichiro ARIMURA
We investigated the possible involvement of Arabidopsis Ca^<2+>-dependent protein kinases (CPKs) and their substrates in the herbivory- or abiotic stress-induced signaling network for the defense-related transcriptional machinery. We found that CP...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(基盤研究(C))
Project Year: 2001 - 2002    Investigator(s): Hiroyuki HORI
We investigated the selenomethionine (SeMet) labeling of two model proteins (GFP and DHFR) by wheat germ in vitro cell-free translation system. The yields of the produced proteins were equal to those of the usual methionine (Met) proteins. We anal...
Studies in mechanism of Plant cell Death
Genome-wide protein functional analysis based on cell-free system
Promotion of Basic Research Activities for Innovative Biosciences
Project Year: 2002 - 2007

Patents

 
US 7674593
10-0911516
US7435538
US7273615