MISC

1999年10月

Biochemical and physiological properties of pedicellarial lectins from the toxopneustid sea urchins

JOURNAL OF NATURAL TOXINS
  • H Nakagawa
  • ,
  • C Yamaguchi
  • ,
  • H Sakai
  • ,
  • K Kanemaru
  • ,
  • H Hayashi
  • ,
  • Y Araki
  • ,
  • Y Tomihara
  • ,
  • M Shinohara
  • ,
  • K Ohura
  • ,
  • H Kitagawa

8
3
開始ページ
297
終了ページ
308
記述言語
英語
掲載種別
出版者・発行元
ALAKEN, INC

Pedicellarial lectins (SUL-I, SUL-II, and TGL-I) were purified from the toxopneustid sea urchins, Toxopneustes pileolus and Tripneustes gratilla using gel filtration chromatography, affinity chromatography, and reverse-phase HPLC. SUL-I (Nakagawa et al., 1996) and SUL-II from the large globiferous pedicellariae of T. pileolus are D-galactose-binding lectins with molecular masses of 32 kDa and 23 kDa, respectively; while TGL-I from the globiferous pedicellariae of T. gratilla is a Ca2+-independent heparin-binding lectin with a molecular mass of 23 kDa. SUL-I induced mitogenic stimulation on murine splenocytes but TGL-I did not. At higher dose ranges SUL-I exhibited inhibitory effects on the cells. The dual response to SUL-I was effectively inhibited by D-galactose. SUL-I enhanced norepinephrine-induced contraction of isolated rat mesenteric artery with endothelium. When endothelium was removed from the artery, acetylcholine did not relax the norepinephrine-induced contraction. In the same artery the enhancing effect of the contraction by SUL-I was abolished, suggesting that SUL-I acts on the endothelium of mesenteric artery, and may release prostanoids. The present results suggest an extracellular function for SUL-I that may have wide-ranging effects in physiological processes. The primary role of pedicellarial lectins from T. pileolus and T. gratilla might be defense against a foreign body.

Web of Science ® 被引用回数 : 10

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000083518700001&DestApp=WOS_CPL
ID情報
  • ISSN : 1058-8108
  • Web of Science ID : WOS:000083518700001

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