2015年6月
Correlation between Fibrillin-1 Degradation and mRNA Downregulation and Myofibroblast Differentiation in Cultured Human Dental Pulp Tissue
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
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- 巻
- 63
- 号
- 6
- 開始ページ
- 438
- 終了ページ
- 448
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1369/0022155415580622
- 出版者・発行元
- SAGE PUBLICATIONS LTD
Myofibroblasts and extracellular matrix are important components in wound healing. Alpha-smooth muscle actin (-SMA) is a marker of myofibroblasts. Fibrillin-1 is a major constituent of microfibrils and an extracellular-regulator of TGF-1, an important cytokine in the transdifferentiation of resident fibroblasts into myofibroblasts. To study the correlation between changes in fibrillin-1 expression and myofibroblast differentiation, we examined alterations in fibrillin-1 and -SMA expression in organotypic cultures of dental pulp in vitro. Extracted healthy human teeth were cut to 1-mm-thick slices and cultured for 7 days. In intact dental pulp, fibrillin-1 was broadly distributed, and -SMA was observed in pericytes and vascular smooth muscle cells. After 7 days of culture, immunostaining for fibrillin-1 became faint concomitant with a downregulation in its mRNA levels. Furthermore, fibroblasts, odontoblasts and Schwann cells were immunoreactive for -SMA with a significant increase in -SMA mRNA expression. Double immunofluorescence staining was positive for pSmad2/3, central mediators of TGF- signaling, and -SMA. The administration of inhibitors for extracellular matrix proteases recovered fibrillin-1 immunostaining; moreover, fibroblasts lost their immunoreactivity for -SMA along with a downregulation in -SMA mRNA. These findings suggest that the expression of -SMA is TGF-1 dependent, and fibrillin-1 degradation and downregulation might be implicated in the differentiation of myofibroblasts in dental pulp wound healing.
- リンク情報
- ID情報
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- DOI : 10.1369/0022155415580622
- ISSN : 0022-1554
- eISSN : 1551-5044
- PubMed ID : 25805839
- Web of Science ID : WOS:000355007000005