2003年8月
Adenoviral transfection of hepatocytes with the thioredoxin gene confers protection against apoptosis and necrosis
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- 巻
- 307
- 号
- 4
- 開始ページ
- 765
- 終了ページ
- 770
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/S0006-291X(03)01253-1
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
A recombinant adenovirus vector containing the human thioredoxin (TRX) gene was constructed using the Cre-loxP recombination system and used to transfect rat hepatocytes with very high efficiency. The TRX gene was expressed in a dose-dependent manner and significantly modulated rat cellular functions. The TRX gene conferred resistance to oxidative stress, such as hydrogen peroxide treatment, on the host hepatocytes. FACS analysis of DNA fragmentation showed that the TRX gene suppressed hepatocyte apoptosis. It also significantly extended the life span of hepatocytes cultured conventionally on polystyrene plates. Liver-specific functions were maintained in the viability-modulated hepatocytes. Moreover, TRX expression did not affect hepatocyte spheroid formation and it extensively suppressed necrosis in the internal cells. Thus, the transfection of hepatocytes with the TRX gene successfully confers global maintenance of liver functions. These findings provide important information for the development of bioartificial liver support systems and gene therapy for liver diseases. (C) 2003 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/S0006-291X(03)01253-1
- ISSN : 0006-291X
- Web of Science ID : WOS:000184510100002