MISC

2003年4月

Sustained expression of Fc-fusion cytokine following in vivo electroporation and mouse strain differences in expression levels

JOURNAL OF BIOCHEMISTRY
  • JJ Jiang
  • ,
  • E Yamato
  • ,
  • J Miyazaki

133
4
開始ページ
423
終了ページ
427
記述言語
英語
掲載種別
DOI
10.1093/jb/mvg055
出版者・発行元
JAPANESE BIOCHEMICAL SOC

We previously demonstrated that cytokine expression following intramuscular gene transfer of a naked plasmid is increased 2 logs by in vivo electroporation, but the relatively low expression levels of the encoded protein is still A limitation for successful gene therapy and gene function studies. We recently reported that the serum viral IL-10 levels achieved by electroporation-mediated intramuscular delivery of pCAGGS-vIL10, a viral IL-10-expressing plasmid, can be further enhanced by modifying the plasmid into an immunoglobulin fusion protein expression plasmid, pCAGGS-vlL10/Fc. Here we examined the applicability of this approach to the expression of an endogenous cytokine, IL-10, in two different inbred mouse strains. We obtained sustained high serum levels of IL-10 in C3H/HeJ mice (C3H), but the level and duration of the gene expression was mouse-strain dependent. Although the serum IL-10 level was also increased by using the IL-10/Fc gene plasmid in C57BL/6 mice (B6), IL-10/Fc and a luciferase reporter showed significantly lower levels in B6 than in C3H mice, and the persistence of pCAGGS-IL10/Fc expression ranged from several days in B6 mice to more than one month in C3H mice. These results suggest that the electroporation-mediated intramuscular delivery of the immunoglobulin fusion protein expression plasmid is simple and very efficient, but mouse strain differences in transgene expression should be taken into consideration in its use.

リンク情報
DOI
https://doi.org/10.1093/jb/mvg055
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000182681500004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/jb/mvg055
  • ISSN : 0021-924X
  • Web of Science ID : WOS:000182681500004

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