MISC

2009年1月

Sohlh2 affects differentiation of KIT positive oocytes and spermatogonia

DEVELOPMENTAL BIOLOGY
  • Shuichi Toyoda
  • ,
  • Tatsushi Miyazaki
  • ,
  • Satsuki Miyazaki
  • ,
  • Takuji Yoshimura
  • ,
  • Mayu Yamamoto
  • ,
  • Fumi Tashiro
  • ,
  • Eiji Yamato
  • ,
  • Jun-ichi Miyazaki

325
1
開始ページ
238
終了ページ
248
記述言語
英語
掲載種別
DOI
10.1016/j.ydbio.2008.10.019
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

The differentiation programs of spermatogenesis and oogenesis are largely independent. In the early stages, however, the mechanisms partly overlap. Here we demonstrated that a germ-cell-specific basic helix-loop-helix (bHLH) transcription factor gene, Sohlh2, is required for early spermatogenesis and oogenesis. SOHLH2 was expressed in mouse spermatogonia from the undifferentiated stage through differentiation and in primordial-to-primary oocytes. Sohlh2-null mice, produced by gene targeting, showed both male and female sterility, owing to the disrupted differentiation of mature (KIT(+)) spermatogonia and oocytes. The Sohlh2-null mice also showed the downregulation of genes involved in spermatogenesis and oogenesis, including the Sohlh1 gene, which is essential for these processes. Furthermore, we showed that SOHLH2 and SOHLH1 could form heterodimers. These observations suggested that SOHLH2 might coordinate with SOHLH1 to control spermatogonial and oocyte genes, including Sohlh1, to promote the differentiation of KIT(+) germ cells in vivo. This study lays the foundation for further dissection of the bHLH network that regulates early spermatogenesis and oogenesis. (C) 2008 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 67

リンク情報
DOI
https://doi.org/10.1016/j.ydbio.2008.10.019
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000262459600023&DestApp=WOS_CPL

エクスポート
BibTeX RIS