MISC

2007年9月

Both Pdx-1 and NeuroD1 genes are requisite for the maintenance of insulin gene expression in ES-derived differentiated cells

DIABETES RESEARCH AND CLINICAL PRACTICE
  • Koichi Saitoh
  • ,
  • Eiji Yamato
  • ,
  • Satsuki Miyazaki
  • ,
  • Jun-Ichi Miyazaki

77
開始ページ
S138
終了ページ
S142
記述言語
英語
掲載種別
DOI
10.1016/j.diabres.2007.01.048
出版者・発行元
ELSEVIER IRELAND LTD

Embryonic stem (ES) cells can differentiate into many cell types. Recent reports have shown that ES cells can differentiate into insulin-producing cells. We have established an ES cell line in which exogenous Pdx-1 expression was precisely regulated by the Tet-off system integrated into the ROSA26 locus and succeeded to produce insulin-producing cells. The Pdx-1 expressing final differentiated insulin-positive cells can be maintained for more than 2 months. However, in spite of their induced expression of Pdx1, the repeated passages of cells lost their capacity to express insulin and NeuroD1 gene. Forced expression of NeuroD1 gene by adenoviral vector in these cells restored the expression of insulin. These results suggested that maintenance of the property of insulin-producing cells defived from ES cells could be achieved by synergistic expression of Pdx-1 and NeuroD1. (c) 2007 Elsevier Ireland Ltd. All rights reserved.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1016/j.diabres.2007.01.048
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000249554200024&DestApp=WOS_CPL

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