MISC

2006年11月10日

Protection against CCl4-induced injury in liver by adenovirally introduced thioredoxin gene

Biochemical and Biophysical Research Communications
  • Katsuhiro Isoda
  • ,
  • Eri Arita
  • ,
  • Midori Kojima
  • ,
  • Masahiro Ikkaku
  • ,
  • Fumi Tashiro
  • ,
  • Eiji Yamato
  • ,
  • Jun-Ichi Miyazaki
  • ,
  • Masaya Kawase
  • ,
  • Masuo Kondoh
  • ,
  • Kiyohito Yagi

350
1
開始ページ
157
終了ページ
161
記述言語
英語
掲載種別
DOI
10.1016/j.bbrc.2006.09.014

Antioxidation therapy is a promising strategy for treating or preventing oxidative stress-related liver diseases. The human thioredoxin (TRX) gene was inserted into an adenovirus vector (Adv-TRX), which was administered to mice. The mice were treated with 1 ml/kg CCl4 48 h after the infection. Blood samples were taken and the liver was excised 24 h after the CCl4 treatment. Serum ammonia, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels were determined, and liver sections were stained with hematoxylin and eosin. RT-PCR analysis showed that the introduced TRX gene was expressed only in the liver. Adv-TRX decreased the serum ammonia, AST, and ALT levels. Hematoxylin-eosin staining indicated that the CCl4-induced injury was significantly prevented by the Adv-TRX infection. The gene delivery of TRX, which plays a central role in intracellular redox control, was shown to be effective in protecting the liver against oxidative stress-induced injury. © 2006 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2006.09.014
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16996028

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