2010
Characterization of Amyloid beta Fibrils with An Aqueous Two-Phase System: Implications of Fibril Formation
SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN
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- Volume
- 17
- Number
- First page
- 121
- Last page
- 128
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.15261/serdj.17.121
- Publisher
- JAPAN ASSOC SOLVENT EXTRACTION
The pathological process of Alzheimer's disease is closely related to amyloid fibril formation by the causative protein, amyloid beta (A beta). The growth behavior of A beta fibrils is predominated by the seeds-monomeric A beta interaction. In this study, the local hydrophobicity of seeds of A beta fibrils was investigated by the aqueous two-phase partitioning method to evaluate the hydrophobic interaction between the seed-monomeric A beta. The seeds showed a high local hydrophobicity relative to the monomer and fibrils. From the fibril growth experiment and the additive effect of Triton X-100, we could demonstrate the contribution of the hydrophobic seeds-monomer interaction to fibril formation.
- Link information
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- DOI
- https://doi.org/10.15261/serdj.17.121
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000279079900011&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=78649673848&origin=inward
- ID information
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- DOI : 10.15261/serdj.17.121
- ISSN : 1341-7215
- SCOPUS ID : 78649673848
- Web of Science ID : WOS:000279079900011