2009年
Characterization of Green Fluorescent Protein Using Aqueous Two-Phase Systems
SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN
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- 巻
- 16
- 号
- 開始ページ
- 145
- 終了ページ
- 150
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- JAPAN ASSOC SOLVENT EXTRACTION
The surface net hydrophobicity and local hydrophobicity of green fluorescent protein was evaluated by using the aqueous two-phase partitioning method. The surface net hydrophobicity of GFP allowed it to be classified as a hydrophobic protein although the local hydrophobicity was low. The local hydrophobicity of the GFP was increased by a pH shift to acidic conditions and was maximal at pH 4. The surface net hydrophobicity increased at acidic conditions less than pH 4. The conformational change of GFP at acidic pH is discussed based on the above results.
- リンク情報
- ID情報
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- ISSN : 1341-7215
- SCOPUS ID : 69549103419
- Web of Science ID : WOS:000267302400016