Apr, 2006
Evaluation of temperature and guanidine hydrochloride-induced protein-liposome interactions by using immobilized liposome chromatography
BIOCHEMICAL ENGINEERING JOURNAL
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- Volume
- 29
- Number
- 3
- First page
- 174
- Last page
- 181
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.bej.2005.08.030
- Publisher
- ELSEVIER SCIENCE SA
Hydrophobic interactions between nine model proteins and net-neutral lipid bilayer membranes (liposomes) under stress conditions were quantitatively examined by using immobilized liposome chromatography (ILC). Small or large unilamellar liposomes were composed of 1-palinitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and immobilized in a gel matrix by utilizing covalent coupling between amino-containing lipids and activated gel beads or avidin-biotin biospecific binding. Retardation of bovine carbonic anhydrase (CAB) in ILC was pronounced at particular temperatures (50 and 60 degrees C) where the local hydrophobicity of theses protein molecules becomes sufficiently large. Protein-induced leakage of a hydrophilic dye (calcein) from immobilized liposomes interior was also drastically enhanced at particular temperatures where large retardation was observed. For other proteins examined, similar results were also observed. The specific capacity factor of the proteins characteristic for the ILC and the amount of calcein released from immobilized liposomes were successfully expressed as a function of the product of the local hydrophobicities of proteins and liposomes, regardless of protein species and the type of the stress conditions applied (denaturant and heating). These findings indicate that lipid membranes have an ability to non-specifically recognize local hydrophobicities of proteins to form stress-mediated supramolecular assemblies with proteins, which may have potential applications in bioprocesses such as protein refolding and separation. ILC was thus found to be a very useful method for the quantitative detection of dynarnic protein-liposome interactions triggered by stress conditions. (c) 2005 Elsevier B.V. All rights reserved.
- Link information
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- DOI
- https://doi.org/10.1016/j.bej.2005.08.030
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000236996400002&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33645992423&origin=inward
- ID information
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- DOI : 10.1016/j.bej.2005.08.030
- ISSN : 1369-703X
- SCOPUS ID : 33645992423
- Web of Science ID : WOS:000236996400002