1998年6月
Selective separation process of proteins based on the heat stress-induced translocation across phospholipid membranes
JOURNAL OF CHROMATOGRAPHY B
- ,
- ,
- 巻
- 711
- 号
- 1-2
- 開始ページ
- 111
- 終了ページ
- 116
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- ELSEVIER SCIENCE BV
Heating of several protein solutions at 40-47 degrees C for 5-60 min in the presence of 1-palmitoyl-2-oleoyI-sn-glycero-3-phosphocholine (POPC) liposomes induced the translocation of beta-galactosidase (beta-gal), alpha-glucosidase (alpha-glu) and bovine carbonic anhydrase (CAB) from outer to inner aqueous phase across the liposome membrane. The translocated amounts of P-gal at various temperatures were maximized under suitable heating conditions (45 degrees C, 30 min). Those of alpha-glu and CAB were maximized at 40-45 and 60 degrees C, respectively. Each maximum value could be correlated with the corresponding local hydrophobicity of each protein evaluated by the aqueous two-phase partitioning method. The possibility to apply these heat-induced translocation phenomena to the bioseparation of proteins was successfully demonstrated for the model mixture solution of P-gal, a-glu and CAB. (C) 1998 Elsevier Science B.V. All rights reserved.
- リンク情報
- ID情報
-
- ISSN : 0378-4347
- Web of Science ID : WOS:000074829800012