MISC

2004年12月

Dielectrophoretic micropatterning with microparticle monolayers covalently linked to glass surfaces

LANGMUIR
  • M Suzuki
  • ,
  • T Yasukawa
  • ,
  • Y Mase
  • ,
  • D Oyamatsu
  • ,
  • H Shiku
  • ,
  • T Matsue

20
25
開始ページ
11005
終了ページ
11011
記述言語
英語
掲載種別
DOI
10.1021/la048111p
出版者・発行元
AMER CHEMICAL SOC

Two-dimensional micropatterns of microparticles were fabricated on glass substrates with negative dielectrophoretic force, and the patterned microparticles were covalently bound on the substrate via cross-linking agents. The line and grid patterns of microparticles were prepared using the repulsive force of negative dielectrophoresis (n-DEP). The template interdigitated microband array (IDA) electrodes (width and gap 50 mum) were incorporated into the dielectrophoretic patterning cell with a fluidic channel. The microstructures on the glass substrates with amino or sulfhydryl groups were immobilized with the cross-linking agents disuccinimidyl suberate (DSS) and m-maleimidobenzoyl-N-hydroxy-succinimide ester (MBS). Diaphorase (Dp), a flavoenzyme, was selectively attached on the patterned microparticles using the maleimide groups of MBS. The enzyme activity on the patterned particles was electrochemically characterized with a scanning electrochemical microscope (SECM) in the presence of NADH and ferrocenylmethanol as a redox mediator. The SECM images proved that Dp was selectively immobilized onto the surface of microparticles to maintain its catalytic activity.

Web of Science ® 被引用回数 : 77

リンク情報
DOI
https://doi.org/10.1021/la048111p
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000225500700030&DestApp=WOS_CPL

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