論文

査読有り 国際誌
2018年8月1日

Screening for long noncoding RNAs associated with oral squamous cell carcinoma reveals the potentially oncogenic actions of DLEU1.

Cell death & disease
  • Koyo Nishiyama
  • Reo Maruyama
  • Takeshi Niinuma
  • Masahiro Kai
  • Hiroshi Kitajima
  • Mutsumi Toyota
  • Yui Hatanaka
  • Tomohiro Igarashi
  • Jun-Ichi Kobayashi
  • Kazuhiro Ogi
  • Hironari Dehari
  • Akihiro Miyazaki
  • Akira Yorozu
  • Eiichiro Yamamoto
  • Masashi Idogawa
  • Yasushi Sasaki
  • Tamotsu Sugai
  • Takashi Tokino
  • Hiroyoshi Hiratsuka
  • Hiromu Suzuki
  • 全て表示

9
8
開始ページ
826
終了ページ
826
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41419-018-0893-2

Recent studies have shown that long noncoding RNAs (lncRNAs) have pivotal roles in human malignancies, although their significance in oral squamous cell carcinoma (OSCC) is not fully understood. In the present study, we identified lncRNAs functionally associated with OSCC. By analyzing RNA-seq datasets obtained from primary head and neck squamous cell carcinoma (HNSCC), we identified 15 lncRNAs aberrantly expressed in cancer tissues. We then validated their expression in 18 OSCC cell lines using qRT-PCR and identified 6 lncRNAs frequently overexpressed in OSCC. Among those, we found that knocking down DLEU1 (deleted in lymphocytic leukemia 1) strongly suppressed OSCC cell proliferation. DLEU1 knockdown also suppressed migration, invasion, and xenograft formation by OSCC cells, which is suggestive of its oncogenic functionality. Microarray analysis revealed that DLEU1 knockdown significantly affects expression of a number of cancer-related genes in OSCC cells, including HAS3, CD44, and TP63, suggesting that DLEU1 regulates HA-CD44 signaling. Expression of DLEU1 was elevated in 71% of primary OSCC tissues, and high DLEU1 expression was associated with shorter overall survival of HNSCC patients. These data suggest that elevated DLEU1 expression contributes to OSCC development, and that DLEU1 may be a useful therapeutic target in OSCC.

リンク情報
DOI
https://doi.org/10.1038/s41419-018-0893-2
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30069008
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070574
ID情報
  • DOI : 10.1038/s41419-018-0893-2
  • PubMed ID : 30069008
  • PubMed Central 記事ID : PMC6070574

エクスポート
BibTeX RIS