1999年2月
Immortal brown adipocytes from p53-knockout mice: Differentiation and expression of uncoupling proteins
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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- 巻
- 255
- 号
- 2
- 開始ページ
- 221
- 終了ページ
- 225
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1006/bbrc.1998.9999
- 出版者・発行元
- ACADEMIC PRESS INC
Brown adipose tissue (BAT) is the specific site for metabolic heat production in mammals. To establish a novel immortal brown adipocyte cell line, the stromal-vascular fraction containing preadipocytes was obtained from interscapular BAT of mice deficient of a tumor-suppressor gene p53. The p53-deficient cells, tentatively named as HB2 cells, could be cultured in vitro after repeated passages and differentiated into adipocytes in the presence of insulin, T-3 and/or troglitazone, expressing some adipocyte-specific genes and accumulating intracellular lipid droplets. The mRNA level of uncoupling protein 1 (UCP1), a mitochondrial protein specifically present in brown adipocytes, was undetectable in HB2 preadipocytes, but increased after adipose differentiation. In HB2 adipocytes, UCP1 mRNA expression was markedly activated after stimulation of the beta-adrenergic receptor pathway. The mRNA of UCP2 and UCP3, recently cloned isoforms of UCP1, were also detected in HB2 adipocytes, but their levels were not influenced by adrenergic stimulation. Thus HB2 cells seem useful for in vitro studies of BAT and UCP functions. (C) 1999 Academic Press.
- リンク情報
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- DOI
- https://doi.org/10.1006/bbrc.1998.9999
- CiNii Articles
- http://ci.nii.ac.jp/naid/80010946692
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/10049689
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000078826000006&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1006/bbrc.1998.9999
- ISSN : 0006-291X
- CiNii Articles ID : 80010946692
- PubMed ID : 10049689
- Web of Science ID : WOS:000078826000006