2019年9月
Development of specific enzyme-linked immunosorbent assays for multiple vitellogenins in marbled sole, Pleuronectes yokohamae
GENERAL AND COMPARATIVE ENDOCRINOLOGY
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- 巻
- 281
- 号
- 開始ページ
- 67
- 終了ページ
- 72
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.ygcen.2019.05.012
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Non-competitive, enzyme-linked immunosorbent assays (ELISAs) for three distinct sole vitellogenins (VtgAa, VtgAb and VtgC) were designed using their purified lipovitellin (Lv) products and corresponding digoxigenin-labeled, anti-Lv polyclonal antibodies, primarily for employment in monitoring estrogenic pollution of the environment. The working range of the ELISAs was from 0.97 to 1,000 ng/mL for all Vtg subtypes. Each ELISA appeared to be specific to the targeted Vtg subtype. Intra- and inter-assay coefficients of variation in the developed ELISAs were lower than 10%. Three Vtg subtypes were induced in serum of immature fish by estradiol-17 beta (E2) injection (0.5 mg/kg body weight). All Vtg subtypes were induced one day after the injection, reaching peak levels (Lv equivalents) within three days, as follows: 39.1 +/- 28.9 mu g/mL (VtgAa), 57.9 +/- 30.7 mu g/mL (VtgAb) and 12.6 +/- 4.8 mu g/mL (VtgC). In wild-caught males, VtgAa, VtgAb and VtgC were detected in ranges from 0.26 to 1.21, 0.19 to 8.69, and 0.17 to 53.50 mu g/mL, respectively, over various sampling periods. In vitellogenic females sampled in January, the average level of VtgAb (8,744.43 +/- 733.93 mu g/mL) was significantly higher than for VtgAa (150.33 +/- 22.35 mu g/mL) or VtgC (57.08 +/- 6.00 mu g/mL); thus VtgAb appeared to be the most dominant Vtg subtype. The present study entails the first report on development of subtype-specific Vtg ELISAs in marbled sole, which empowers us to detect and monitor estrogenic contamination in aquatic environments inhabited by this species.
- リンク情報
- ID情報
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- DOI : 10.1016/j.ygcen.2019.05.012
- ISSN : 0016-6480
- eISSN : 1095-6840
- PubMed ID : 31112714
- Web of Science ID : WOS:000479327500008