1996年5月15日
Effects of glucosylceramide synthase inhibitor and ganglioside GQ1b on synchronous oscillations of intracellular Ca2+ in cultured cortical neurons.
Biochemical and biophysical research communications
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- 巻
- 222
- 号
- 2
- 開始ページ
- 494
- 終了ページ
- 8
- 記述言語
- 英語
- 掲載種別
- Scientific Journal
- DOI
- 10.1006/bbrc.1996.0772
To evaluate the role of endogenous gangliosides in synapse formation, the glucosylceramide synthase inhibitor, D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP), was used to deplete glycosphingolipids (GSLs) of cultured rat cerebral cortical neurons. Synapse formation between the neurons was estimated by the synchronous oscillation of synaptic activity monitored by fura-2 calcium imaging. Treatment with D-PDMP resulted in dose- and time-dependent decreases in the frequency of synchronous oscillations. When a series of gangliosides (GM3, GM1, GD3, GD1b, GT1b, and GQ1b) was supplemented to the GSL depleted cells, only GQ1b was able to normalize the decreased frequency by D-PDMP. These results suggest that de novo synthesis of a particular molecular species of the gangliosides, GQ1b, is essential for synapse formation and synaptic activity.
- リンク情報
- ID情報
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- DOI : 10.1006/bbrc.1996.0772
- ISSN : 0006-291X
- CiNii Articles ID : 80008991432
- PubMed ID : 8670233