2018年
Pharmacological Modulation of Glycosphingolipid Metabolism.
Methods in molecular biology (Clifton, N.J.)
- ,
- ,
- 巻
- 1804
- 号
- 開始ページ
- 401
- 終了ページ
- 410
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1007/978-1-4939-8552-4_19
The experimental approach to deplete cellular glycosphingolipids (GSLs) with the specific inhibitors of glycosphingolipid biosynthesis has the potential to identify functions of endogenous GSLs. Most GSLs are derived from glucosylceramide (GlcCer). D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (D-PDMP) inhibits GIcCer synthase and has been used extensively to study the biological functions of living cells. D-PDMP inhibits mTORC1 activity, which is independent of its inhibitory activity on GlcCer synthase. We also developed an analog of D-PDMP, D-threo-1-phenyl-2-benzyloxycarbonylamino-3-pyrrolidino-1-propanol (D-PBPP) lacking the effect on mTORC1. Here, we summarize the effects of D-PDMP and D-PBPP on the metabolism of GSLs and cell growth.
- リンク情報
- ID情報
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- DOI : 10.1007/978-1-4939-8552-4_19
- PubMed ID : 29926420