2012年7月
Amphotericin B Up-regulates Lipid A-induced IL-6 Production via Caspase-8
JOURNAL OF DENTAL RESEARCH
- ,
- ,
- 巻
- 91
- 号
- 7
- 開始ページ
- 709
- 終了ページ
- 714
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1177/0022034512446486
- 出版者・発行元
- SAGE PUBLICATIONS INC
Amphotericin B, an antifungal drug used to treat candidiasis, has been reported to induce pro-inflammatory cytokine production in cultured cells. This study investigated the effects of amphotericin B on pro-inflammatory cytokine production in response to lipid A, the bioactive component of lipopolysaccharide (LPS) in the cell walls of Gram-negative bacteria. Amphotericin B alone elicited a slight increase in interleukin (IL)-6 and IL-8 production by human gingival fibroblasts. However, amphotericin B synergistically up-regulated lipid A-induced production of IL-6 and IL-8. While amphotericin B minimally activated nuclear factor (NF)-kappa B, it synergistically increased lipid A-induced NF-kappa B activation. Pre-treatment with methyl-beta-cyclodextrin (M beta CD), a cholesterol-binding agent, reduced IL-6 and IL-8 production in human gingival fibroblasts. Cholesterol-saturated M beta CD also reversed cytokine production, suggesting that the synergistic production of cytokines by amphotericin B and lipid A is dependent on cholesterol-rich microdomains. Amphotericin B activated caspase-8. In addition, a caspase-8 inhibitor inhibited IL-6 production by amphotericin B and lipid A. This suggests that caspase-8 is required for the synergistic production of IL-6 by amphotericin B and lipid A. Collectively, our results suggest that periodontal treatment carried out before amphotericin B treatment may protect against lipid A-induced pro-inflammatory cytokine production.
- リンク情報
-
- DOI
- https://doi.org/10.1177/0022034512446486
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22538414
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000305708600013&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84864128234&origin=inward
- ID情報
-
- DOI : 10.1177/0022034512446486
- ISSN : 0022-0345
- eISSN : 1544-0591
- PubMed ID : 22538414
- SCOPUS ID : 84864128234
- Web of Science ID : WOS:000305708600013