ISHIDA Hiroyuki

J-GLOBAL         Last updated: Mar 8, 2019 at 11:04
ISHIDA Hiroyuki
Tohoku University
Graduate School of Agriculture Division of Life Science
Job title
Associate Professor
農学博士(Tohoku University)
Research funding number

Research Interests


Research Areas


Academic & Professional Experience

Apr 2009
准教授, 東北大学大学院農学研究科
Apr 2007
Mar 2009
助教 助教, 東北大学大学院農学研究科
Apr 1999
Mar 2007
東北大学大学院農学研究科 助手

Committee Memberships

Mar 2017
Saclay Plant Sciences  書面審査委員
Mar 2016
Saclay Plant Sciences  書面審査委員
Mar 2016
United States-Israel Binational Science Foundation  書面審査委員
Oct 2015
米国植物生物学会  Plant Physiology 論文審査委員
Mar 2015
日本植物生理学会  Plant Cell Physiology 論文審査委員

Awards & Honors

Jul 2007
当該会議における全669題の発表のうち特に内容が優れていた7題に授与された, 第14回国際光合成会議ベストポスター賞, International Society of Photosynthesis Research

Published Papers

Masanori Izumi, Hiroyuki Ishida*
Plant signaling & behavior   14 1552057   Jan 2019   [Refereed]
本人がCorresponding author(*)である。
Takaaki Hirota, Masanori Izumi, Masanori Izumi, Masanori Izumi, Shinya Wada, Shinya Wada, Amane Makino, Hiroyuki Ishida*
Plant and Cell Physiology   59 1363-1376   Jul 2018
本人がCorresponding author(*)である。
Sakuya Nakamura, Jun Hidema, Wataru Sakamoto, Hiroyuki Ishida, Masanori Izumi, Masanori Izumi, Masanori Izumi
Plant Physiology   177 1007-1026   Jun 2018
Hiroyuki Ishida, Amane Makino
Soil Science and Plant Nutrition   64 100-105   Jan 2018
本人がCorresponding author(*)である。
Masatake Eguchi, Kazuhiko Kimura, Amane Makino, Hiroyuki Ishida
Soil Science and Plant Nutrition   63 342-350   Jul 2017
© 2017 Japanese Society of Soil Science and Plant Nutrition. Autophagy is a degradation system for cellular components conserved in eukaryotes. In Arabidopsis, it is known that autophagy is crucial for growth under dark-induced carbon starvation a...
Wada, S., Hayashida, Y., Izumi, M., Kurusu, T., Hanamata, S., Kanno, K., Kojima, S., Yamaya, T., Kuchitsu, K., Makino, A., Ishida, H.*
Plant Physiol.   168 60-73   May 2015   [Refereed]
本人がCorresponding author(*)である。
Masanori Izumi, Masanori Izumi, Jun Hidema, Shinya Wada, Eri Kondo, Takamitsu Kurusu, Takamitsu Kurusu, Kazuyuki Kuchitsu, Amane Makino, Amane Makino, Amane Makino, Hiroyuki Ishida
Plant Physiology   167 1307-1320   Jan 2015
本人がCorresponding author(*)である。
石田 宏幸
化学と生物   52 610-615   Sep 2014
Ishida, H.*, Izumi, M., Wada, S., Makino, A.
Biochim. Biophys. Acta-Bioenerg.   1837 512-521   Apr 2014   [Refereed][Invited]
本人がCorresponding authorである
Takamitsu Kurusu, Takamitsu Kurusu, Takamitsu Kurusu, Tomoko Koyano, Shigeru Hanamata, Takahiko Kubo, Yuhei Noguchi, Chikako Yagi, Noriko Nagata, Takashi Yamamoto, Takayuki Ohnishi, Yozo Okazaki, Nobutaka Kitahata, Daichi Ando, Masaya Ishikawa, Shinya Wada, Akio Miyao, Hirohiko Hirochika, Hiroaki Shimada, Hiroaki Shimada, Amane Makino, Kazuki Saito, Kazuki Saito, Hiroyuki Ishida, Tetsu Kinoshita, Nori Kurata, Kazuyuki Kuchitsu, Kazuyuki Kuchitsu
Autophagy   10 878-888   Jan 2014
In flowering plants, the tapetum, the innermost layer of the anther, provides both nutrient and lipid components to developing microspores, pollen grains, and the pollen coat. Though the programmed cell death of the tapetum is one of the most crit...
Yuki Ono, Shinya Wada, Masanori Izumi, Amane Makino, Amane Makino, Hiroyuki Ishida
Plant, Cell and Environment   36 1147-1159   Jun 2013
During leaf senescence, Rubisco is gradually degraded and its components are recycled within the plant. Although Rubisco can be mobilized to the vacuole by autophagy via specific autophagic bodies, the importance of this process in Rubisco degrada...
Wada, S., Ishida, H. *, Izumi, M., Yoshimoto, K., Ohsumi, Y., Mae, T., Makino, A.
Plant Physiology   149    Feb 2009   [Refereed]
本人がCorresponding author(*)である。IF = 6.4
Ishida, H. *, Yoshimoto, K., Izumi, M., Reisen, D., Yano, Y., Makino, A., Ohsumi, Y., Hanson, M.R., Mae, T.
Plant Physiology   148 142-155   Sep 2008   [Refereed]
本人がCorresponding author(*)である。IF=6.4
Izumi, M, Ishida, H., Nakamura, S., Hidema, J.
Plant Cell   29 377-394   Feb 2017   [Refereed]
Klionsky, D. and others (Ishida, H.をアルファベット順で含む)
Autophagy   12 1-122   Jan 2016   [Refereed]
Izumi, M., Hidema, J., Ishida, H.*
Plant Signal Behav.   10 e1101199   Oct 2015   [Refereed]
本人がCorresponding authorである
Kurusu, T., Koyano, T., Hanamata, S., Kubo, T., Noguchi, Y., Yagi, C., Nagata, N., Yamamoto, T., Ohnishi., T, Okazaki, Y., Kitahata, N., Ando, D., Ishikawa, M., Wada, S., Miyao, A., Hirochika, H., Shimada, H., Makino, A., Saito, K., Ishida, H., Kinoshita, T., Kurata, N., Kuchitsu, K.
Autophagy   10(5) in press-in press   Mar 2014   [Refereed]
インパクトファクターが「10以上」である(IF =12.1)
Ono, Y.,Wada, S., Izumi, M., Makino, A., Ishida, H*
Plant Cell Environ.   36 1147-159   May 2013   [Refereed]
本人がCorresponding authorである
Impact factorが5.0以上である
Izumi, M., Hidema, J., Makino, A., Ishida, H.*
Plant Signal Behav.   -20130000   Apr 2013   [Refereed]
本人がCorresponding authorである
Izumi, M., Hidema, J., Makino, A., Ishida, H.*
Plant Physiol.   161 1682-1693   Apr 2013   [Refereed]
本人がCorresponding authorである
Klionsky, D et al.(アルファベット順でIshida, H.含む)
Autophagy   12 1-222   Apr 2012   [Refereed]
impact factorが10以上である
Guidelines for the use and interpretation of assays for monitoring autophagy
Klionsky, D et al.(アルファベット順でIshida, H.含む)
Autophagy   8 1-100   Apr 2012   [Refereed]
impact factorが5以上である
Nakayama, M, Kaneko, Y., Miyazawa, Y., Fujii N., Higashitani, N., Wada, S., Ishida, H., Yoshimoto, K., Shirasu, K., Yamada, K., Nishimura, M., Takahashi, H
Planta      Apr 2012   [Refereed]
impact factor: 3.1
Izumi, M., Tsunoda, H., Suzuki, Y., Makino, A., Ishida, H*
J. Exp. Bot.   63 2159-2170   Jan 2012   [Refereed]
本人がCorresponding authorである
5-Yr impact factor: 5.0
Takahashi, M., Teranishi, M., Ishida, H., Kawasaki, J., Takeuchi1, A., Yamaya, T., Watanabe, M., Makino, A., Hidema, J.*
Plant J.   66 433-442   May 2011   [Refereed]
インパクトファクター = 6.9
Izumi, M., Ishida, H.*
Plant Signal Behav.   6    May 2011   [Refereed][Invited]
インパクトファクター = 2.0
本人がCorresponging authorである
Izumi, M., Wada, S., Makino, A., Ishida, H.*
Plant Physiol.   154 1196-1209   Nov 2010   [Refereed]
インパクトファクター: 6.2
本人がCorresponding authorである
Nakano R., Ishida H.*, Kobayashi M., Makino A., Mae T.
Plant Biol.   12 35-45   Jan 2010   [Refereed]
本人がCorresponding author(*)である。インパクトファクター(IF)=2.0
Irving, L.J., Suzuki, Y., Ishida, H., Makino, A.
Advances in Botanical Research   54 139-182   2010   [Refereed][Invited]
Rubisco turnover and nitrogen in a leaf
Ishida, H., Suzuki, Y., Makino A.
Nitorgen assimilation in plants   277-285   2010   [Invited]
The chloroplast degradation in two different pathways in individually darkened leaves of Arabidopsis by autophagy
Wada S., Ishida H., Yoshimoto K., Ohsumi Y., Makino A.
Autophagy      Sep 2009
The analysis of factors affecting the creation of Rubisco-containing bodies(RCBs), a specific autophagic process for chloroplast degradation in plants
Izumi M., Ishida H., Makino A.
Autophagy      Sep 2009
The role of autophagy in nutrient starvation and aging
Yoshimoto K., Ishida H., Wada S., Ohsumi Y., Shirasu K.
Autophagy      Sep 2009   [Invited]
Ishida H.*, Wada S.
Autophagy   5 736-737   Jul 2009   [Refereed][Invited]
本人が筆頭著者かつCorresponding author(*)である。インパクファクター (IF) =6.4
Wada S., Ishida H.*
Plant Signal Behav.   4 565-567   Jun 2009   [Refereed][Invited]
本人がCorresponding author (*)である。
Autophagy of chloroplast proteins
Ishida H., Wada S.
Photosynthesis research (Japanese)   53 89-94   Dec 2008   [Invited]
Chloroplasts are partially mobilized to the vacuole by autophagy
Ishida, H.*, Yoshimoto, K.
Autophagy   4 961-962   Oct 2008   [Refereed][Invited]
本人がCorresponding author(*)である。IF=4.7
Visualization of Rubisco-containing bodies derived from chloroplasts in living cells of Arabidopsis.
Ishida, H., Yoshimoto, K., Reisen, D., Makino, A., Ohsumi, Y., Hanson, M.R., Mae, T.
Photosynthesis. Energy from the sun   1207-1210   Sep 2008   [Refereed]
Rubisco and photosynthesis in cereal crops
Makino, A., Ishida, H.
Tohoku Journal of Agricultural Research   58 127-135   2008   [Invited]
Xu Y, Ishida H, Reisen D, Hanson MR.
BMC Plant Biol.   13(6) 8   Apr 2006   [Refereed]
IF: 3.4 (2006年、新しい雑誌のため出版社独自の算出値)
Nakano R., Ishida H., Makino A., Mae T.
Plant Cell Physiol.   47 270-276   Feb 2006   [Refereed]
IF: 3.2 (2005年)
Fragmentation of the large subunit of ribulose-1,5-bisphosphate carboxylase by reactive oxygen species occurs in vivo
Nakano R., Ishida H., Makino A., Mae T.
Photosynthesis: Fundamental Aspects to Global Perspectives   (1) 509-510   2005
Ribulose-1,5-bisphosphate carboxylase/oxygenase is excluded from chloroplasts by specific bodies in senescing wheat and rice leaves
Ishida H., Chiba A., Hikichi R., Nishizawa N.K., Makino A., Mae T.
Photosynthesis: Fundamental Aspects to Global Perspectives   (2) 837-838   2005
石田 宏幸
日本光合成研究会会報   (41) 22-24   Nov 2004   [Invited]
Chiba A., Ishida H., Nishizawa N.K., Makino A., Mae T.
Plant Cell Physiol.   44 914-921   Sep 2003   [Refereed]
Luo S., Ishida H., Makino A., Mae T.
J. Biol. Chem.   277 12382   2002   [Refereed]
Ishida H., Anzawa D., Kokubun N., Makino A., Mae T.
Plant Cell Environ.   25 625   2002   [Refereed]
Ishida H., Makino A., Mae T
J. Biol. Chem.   274 5222-5226   Feb 1999   [Refereed]
Light-dependent fragmentation of ribulose-1,5-bisphosphate carboxylase/oxygenase in chloroplasts isolated from wheat leaves
Ishida H., Shimizu S., Makino A., Mae T.
Photosynthesis: Mechanisms and Effects   (3) 2249-2252   1998
Determination of the cleavage site of the large subunit of Rubisco by active oxygen species and its inhibition by CABP in the lysates of wheat chloroplasts
Ishida H., Makino A., Mae T.
Photosynthesis: Mechanisms and Effects   (3) 2245-2248   1998
The large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase is fragmented into 37-kDa and 16-kDa polypeptides by active oxygen in the lysates of chloroplasts from primary leaves of wheat
Ishida H., Nishimori Y., Sugisawa M., Makino A., Mae T
Plant Cell Physiol.   38 471-479   Apr 1997   [Refereed]


Hiroyoshi Matsumura, Eiichi Mizohata, Hiroyuki Ishida, Ayako Kogami, Takeshi Ueno, Amane Makino, Tsuyoshi Inoue, Akiho Yokota, Tadahiko Mae, Yasushi Kai, Yasushi Kai
Journal of Molecular Biology   422 75-86   Sep 2012
The key enzyme of plant photosynthesis, d-ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco), must be activated to become catalytically competent via the carbamylation of Lys201 of the large subunit and subsequent stabilization by Mg2+coord...
Masanori Izumi, Jun Hidema, Hiroyuki Ishida
Plant Signaling and Behavior   8    Aug 2013
Autophagy is an intracellular process leading to vacuolar degradation of cytoplasmic components, which is important for nutrient recycling. Autophagic degradation of chloroplastic proteins via Rubisco-containing bodies is activated in leaves upon ...
Masanori Izumi, Masanori Izumi, Jun Hidema, Hiroyuki Ishida
Plant Signaling and Behavior   10    Jan 2015
© Masanori Izumi, Jun Hidema, and Hiroyuki Ishida. Autophagy is an evolutionarily conserved process leading to the degradation of intracellular components in eukaryotes, which is important for nutrient recycling especially in response to starvatio...
Masanori Izumi, Honami Tsunoda, Yuji Suzuki, Amane Makino, Hiroyuki Ishida
Journal of Experimental Botany   63 2159-2170   Mar 2012
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit (RBCS) is encoded by a nuclear RBCS multigene family in many plant species. The contribution of the RBCS multigenes to accumulation of Rubisco holoenzyme and photosynthetic ch...
Masaaki Takahashi, Mika Teranishi, Hiroyuki Ishida, Junji Kawasaki, Atsuko Takeuchi, Tomoyuki Yamaya, Masao Watanabe, Amane Makino, Jun Hidema
Plant Journal   66 433-442   May 2011
Plants use sunlight as energy for photosynthesis; however, plant DNA is exposed to the harmful effects of ultraviolet-B (UV-B) radiation (280-320 nm) in the process. UV-B radiation damages nuclear, chloroplast and mitochondrial DNA by the formatio...
Shen Luo, Hiroyuki Ishida, Amane Makino, Tadahiko Mae
Journal of Biological Chemistry   277 12382-12387   Apr 2002
Previous work has demonstrated that the large subunit (rbcL) of ribulose 1,5-bisphosphate carboxylase/oxgenase (RuBisCo) from wheat is cleaved at Gly-329 by the Fe2+/ascorbatefH2O2 system (Ishida, H., Makino, A.,and Mae, T. (1999) J. Biol. Chem. 2...
Hiroyuki Ishida, Amane Makino, Mae Tadahiko, Mae Tadahiko
Journal of Biological Chemistry   274 5222-5226   Feb 1999
The large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) in the illuminated lysates of wheat (Triticum aestivum L.) chloroplasts is broken down by reactive oxygen radicals into 37- and 16-kDa polypeptides. Analysis of t...
Light stress and Rubisco degradation
石田 宏幸、牧野 周、前 忠彦
kagaku to seibutsu   38 8-9   Jan 2000
Hiroyuki Ishida, Yoshito Nishimori, Miki Sugisawa, Amane Makino, Tadahiko Mae
Plant and Cell Physiology   38 471-479   Jan 1997
Lysates of chloroplasts isolated from wheat (Triticum aestivum L. cv. Aoba) leaves were incubated on ice (pH 5.7) for 0 to 60 min in light (15 μnol quanta m-2s-1), and degradation of the large subunit (LSU) of ribulose-1,5-bisphosphate carboxylase...
Tomoyuki Yamaya, Hirohiko Ishida, Kazunari Kamachi, Kunihiko Ojima
Plant and Cell Physiology   31 325-331   Dec 1990
A methionine sulfoximine (MSX) tolerant cell line of tobacco (Nicotiana tabacum L. cv. Xanthi) cells was selected by culturing the wild-type cells in suspension media in the presence of MSX at a step-wise increase in its concentration (0.3 to 5 μM...
Rubisco: Photosynthesis and its protein turnover
Mae, T, Makino, A., Ishida, H.
Applied Biological Science.   5 79-87   1999

Conference Activities & Talks

Autophagy and nutrient recycling in plants
Finnish-Japanese symposium 2016   5 Sep 2016   
Autophagy provides substrates to amino acid catabolic pathways as an adaptive response to sugar starvation in Arabidopsis
The 17th International Congress on Photosynthesis Research   7 Aug 2016   
Autophagy of chloroplasts as an adaptive response to sugar starvation in Arabidopsis
International Conference on Arabidopsis   29 Jun 2016   
Chloroplast autophagy as an adaptive response to sugar starvation in Arabidopsis
Gordon Research conference ‘Mitochondria and Chloroplasts'   19 Jun 2016   
中村咲耶, 泉正範, 石田宏幸, 日出間純
日本植物生理学会   18 Mar 2016   

Research Grants & Projects

Molecular Mechanisms of Degradation of chloroplast proteins during leaf senescence
Basic Science Research Program
Project Year: Apr 1999   

Social Contribution

日本学術振興会 特別研究員等審査会委員・専門委員
[Others]  1 Aug 2015 - 31 Jul 2017
日本学術振興会 特別研究員等の採用にかかる書面審査を行う
米国 科学研究費審査員
[Others]  Aug 2009 - Oct 2010
米国 科学研究費審査員
[Others]  Aug 2008 - Oct 2008
オートファジーを可視化 イネ葉緑体の分解過程解明
[Informant]  科学新聞  24 Apr 2015
イネの葉緑体分解 追跡 東北大など 自食作用が関与
[Informant]  日経産業新聞  16 Apr 2015


Apr 2008   東北大学、学際科学国際高等研究センター「領域創成研究」