MISC

2006年1月

p53 protein transduction therapy: Successful targeting and inhibition of the growth of the bladder cancer cells

EUROPEAN UROLOGY
  • M Inoue
  • ,
  • K Tomizawa
  • ,
  • M Matsushita
  • ,
  • YF Lu
  • ,
  • T Yokoyama
  • ,
  • H Yanai
  • ,
  • A Takashima
  • ,
  • H Kumon
  • ,
  • H Matsui

49
1
開始ページ
161
終了ページ
168
記述言語
英語
掲載種別
DOI
10.1016/j.eururo.2005.08.019
出版者・発行元
ELSEVIER SCIENCE BV

Introduction: Virus-mediated gene therapy for bladder cancer has some problems, such as efficiency of gene delivery and safety issues. We have reported that poly-arginine peptide (11R) has the ability to increase protein transduction in cells. Here, we show that p53 protein transduction using 11R. is useful for targeting to bladder tumors and suppressing the growth of bladder cancer cells.
Materials and methods: An 11R-fused p53 protein (11R-p53) was transduced in bladder cancer cell lines (J82 and T24) to evaluate the anti-tumor effect. Cell viability was assessed by performing the 4- [3-(4-iodophenyl) 2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate (WST) assay. To investigate whether 11R-p53 enhanced the effect on anti-cancer drug-dependent apoptosis of bladder cancer cells, the cell lines were cotreated with 11R-p53 and cis-diaminedichloroplatinum (CDDP). Apoptotic cells were identified using Hoechst staining. To investigate the efficiency of protein transduction mediated by 11R in bladder tumors in vivo, SCID mice were transplanted with J82 cells in the bladder and 11R-GFP was transurethrally transduced into the bladder. The transduction of 11R-GFP in the tumor was examined by confocal microscopy.
Results: 11R-p53 inhibited the growth of both J82 and T24 cells in a dose-dependent manner. The transduction of 11R-p53 enhanced CDDP-dependent induction of apoptosis. Transurethral application of 11R-GFP resulted in transduction of GFP in bladder tumors but not in the normal bladder epithelium or subepithelial tissues.
Conclusion: The present results suggest that p53 protein transduction therapy may be a promising method for the treatment of bladder cancer. (c) 2005 Elsevier B.V. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.eururo.2005.08.019
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000234576900025&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.eururo.2005.08.019
  • ISSN : 0302-2838
  • Web of Science ID : WOS:000234576900025

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