2000年
Genetic Evaluation of Peroxisomal and Cytosolic Acetoacetyl-CoA Thiolase Isozymes in n-Alkane-Assimilating Diploid Yeast, Candida tropicalis
Cell Biochemistry and Biophysics
- ,
- ,
- 巻
- 32
- 号
- 開始ページ
- 285
- 終了ページ
- 290
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1385/CBB:32:1-3:285
- 出版者・発行元
- Humana Press
The n-alkane-assimilating diploid yeast, Candida tropicalis, possesses two acetoacetyl-CoA thiolase (Thiolase I) isozymes encoded by one allele: peroxisomal and cytosolic Thiolase Is encoded by both CT-T1A and CT-T1B. To clarify the function of peroxisomal and cytosolic Thiolase Is, the site-directed mutation leading Thiolase I ΔC6 without a putative C-terminal peroxisomal targeting signal was introduced on CT-T1A locus in the ct-t1bΔ-null mutant. The C-terminus-truncated Thiolase I was active and solely present in the cytosol. Although the ct-t1aΔ/t1bΔ-null mutants showed mevalonate auxotrophy, the mutants having the C-terminus-truncated Thiolase I did not require mevalonate for growth, as did the strains having cytosolic Thiolase I. These results demonstrate that the presence of Thiolase I in the cytoplasm is indispensable for the sterol synthesis in this yeast. It is of greater interest that peroxisomal and cytosolic Thiolase I isozymes, products of the same genes, play different roles in the respective compartments, although further investigations will be necessary to analyze how to be sorted into peroxisomes and the cytosol.
- リンク情報
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- DOI
- https://doi.org/10.1385/CBB:32:1-3:285
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/11330060
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000088316300032&DestApp=WOS_CPL
- URL
- http://orcid.org/0000-0003-1778-2005
- ID情報
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- DOI : 10.1385/CBB:32:1-3:285
- ISSN : 1085-9195
- ORCIDのPut Code : 28821049
- PubMed ID : 11330060
- SCOPUS ID : 0034571517
- Web of Science ID : WOS:000088316300032