Proceedings of the National Academy of Sciences of the United States of America 114 5641-5646 May 2017
Cold temperatures lead to nullification of circadian rhythms in many organisms. Two typical scenarios explain the disappearance of rhythmicity: The first is oscillation death, which is the transition from self-sustained oscillation to damped oscil...
BACKGROUND:Most organisms, especially photoautotrophs, alter their behaviours in response to day-night alternations adaptively because of their great reliance on light. Upon light-to-dark transition, dramatic and universal decreases in transcripti...
J. Ishihara, J. Ishihara, M. Tachikawa, A. Mochizuki, Y. Sako, H. Iwasaki, S. Morita
Proceedings of SPIE - The International Society for Optical Engineering 8879 Sep 2013
The objective of our research was to predict cell fates of a multicellular system, accompanied by cellular differentiation. To fulfill this objective, we sought to distinguish the differentiated and undifferentiated cells of filamentous cyanobacte...
Diazotrophic heterocyst formation in the filamentous cyanobacterium, Anabaena sp. PCC 7120, is one of the simplest pattern formations known to occur in cell differentiation. Most previous studies on heterocyst patterning were based on statistical ...
Proceedings of the National Academy of Sciences of the United States of America 106 14168-14173 Aug 2009
In the unicellular cyanobacterium Synechococcus elongatus PCC 7942, essentially all promoter activities are under the control of the circadian clock under continuous light (LL) conditions. Here, we used high-density oligonucleotide arrays to explo...
The use of synthetic biology to design artificial gene circuits is an important approach for understanding the principles underlying the complicated dynamic behaviours of biomolecular networks, such as genetic switching and biological rhythms. The...
Shannon R. Mackey, Shannon R. Mackey, Jong Soon Choi, Jong Soon Choi, Yohko Kitayama, Hideo Iwasaki, Guogang Dong, Susan S. Golden, Susan S. Golden
Journal of Bacteriology 190 3738-3746 May 2008
Diverse organisms time their cellular activities to occur at distinct phases of Earth's solar day, not through the direct regulation of these processes by light and darkness but rather through the use of an internal biological (circadian) clock th...
Journal of Biological Chemistry 282 36887-36894 Dec 2007
Among the σ70 family bacterial σ factors, group 2 σ factors have similar promoter recognition specificity to group 1 (principal) σ factors and express and function under specific environmental and physiological conditions. In general, the cyanobac...
In the cyanobacterium, Synechococcus elongatus, most promoters are regulated by a circadian clock under continuous light (LL) conditions. Nevertheless, the basic circadian oscillation is primarily generated by alternating KaiC phosphorylation/deph...
Semi-synthetic analysis toward validating roles of a Transcription/Translation feedback process for the cyanobacterial KaiC-protein-based circadian system in Escherichia coli
Proceedings of the National Academy of Sciences of the United States of America 103 12109-12114 Aug 2006
KaiA, KaiB, and KaiC clock proteins from cyanobacteria and ATP are sufficient to reconstitute the KaiC phosphorylation rhythm in vitro, whereas almost all gene promoters are under the control of the circadian clock. The mechanism by which the KaiC...
KaiA, KaiB, and KaiC are essential proteins of the circadian clock in the cyanobacterium Synechococcus elongatus PCC 7942. The phosphorylation cycle of KaiC that occurs in vitro after mixing the three proteins and ATP is thought to be the master o...
Histidine Kinases in Signal Transduction 297-311 Nov 2002
Circadian rhythms, endogenous biological oscillations with a period of about a day, have been observed in innumerable physiological processes in most of organisms from cyanobacteria to higher plants and mammals. These rhythms are thought to be ada...
H. Iwasaki, H. Iwasaki, M. Takahashi, T. Niimi, T. Niimi, O. Yamashita, T. Yaginuma, T. Yaginuma
Insect Molecular Biology 6 131-141 Jan 1997
In diapausing eggs of the silkworm, Bombyx mori, embryonic cells are arrested at G2phase. The ability to undertake cell division is resumed in the course of dispause termination caused by such a treatment as acclimation to 5°C. As an initial trial...
藍色細菌の概日時計
岩崎秀雄,石浦正寛,近藤孝男
日本時間生物学会会誌 3(1) 6-24 1997
Cloning of cDNAs encoding Bombyx homologues of Cdc2 and Cdc2-related kinase from eggs.
H Iwasaki, M Takahashi, T Niimi, O Yamashita, T Yaginuma
15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 1 Dec 2011
We designed and fabricated a microfluidic system, which has 128 microchannels for observation of muliticellular filamentous cyanobacteria, Anabaena sp. PCC 7120, under microscope for a long term with controlled perfusion culture. Using automated p...
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