2003年5月
KaiB functions as an attenuator of KaiC phosphorylation in the cyanobacterial circadian clock system
EMBO JOURNAL
- ,
- ,
- ,
- 巻
- 22
- 号
- 9
- 開始ページ
- 2127
- 終了ページ
- 2134
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/emboj/cdg212
- 出版者・発行元
- OXFORD UNIV PRESS
In the cyanobacterium Synechococcus elongatus PCC 7942, the KaiA, KaiB and KaiC proteins are essential for generation of circadian rhythms. We quantitatively analyzed the intracellular dynamics of these proteins and found a circadian rhythm in the membrane/cytosolic localization of KaiB, such that KaiB interacts with a KaiA-KaiC complex during the late subjective night. KaiB-KaiC binding is accompanied by a dramatic reduction in KaiC phosphorylation and followed by dissociation of the clock protein complex(es). KaiB attenuated KaiA-enhanced phosphorylation both in vitro and in vivo. Based on these results, we propose a novel role for KaiB in a regulatory link among subcellular localization, protein-protein interactions and post-translational modification of Kai proteins in the cyanobacterial clock system.
- リンク情報
- ID情報
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- DOI : 10.1093/emboj/cdg212
- ISSN : 0261-4189
- Web of Science ID : WOS:000182630900019