2012年
RpaB, another response regulator operating circadian clock-dependent transcriptional regulation in Synechococcus elongatus PCC 7942
Journal of Biological Chemistry
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- 巻
- 287
- 号
- 31
- 開始ページ
- 26321
- 終了ページ
- 26327
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1074/jbc.M111.338251
- 出版者・発行元
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
The circadian clock of cyanobacteria is composed of KaiA, KaiB, and KaiC proteins, and the SasA-RpaA two-component system has been implicated in the regulation of one of the output pathways of the clock. In this study, we show that another response regulator that is essential for viability, the RpaA paralog, RpaB, plays a central role in the transcriptional oscillation of clock-regulated genes. In vivo and in vitro analyses revealed that RpaB and not RpaA could specifically bind to the kaiBC promoter, possibly repressing transcription during subjective night. This suggested that binding may be terminated by RpaA to activate gene transcription during subjective day. Moreover, we found that rpoD6 and sigF2, which encode group-2 and group-3 sigma factors forRNApolymerase, respectively, were also targets of the RpaAB system, suggesting that a specific group of sigma factors can propagate genome-wide transcriptional oscillation. Our findings thus reveal a novel mechanism for a circadian output pathway that is mediated by two paralogous response regulators.
- リンク情報
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- DOI
- https://doi.org/10.1074/jbc.M111.338251
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22665493
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000306916300058&DestApp=WOS_CPL
- Scopus Url
- http://www.scopus.com/inward/record.url?eid=2-s2.0-84864392477&partnerID=MN8TOARS
- ID情報
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- DOI : 10.1074/jbc.M111.338251
- ISSN : 0021-9258
- eISSN : 1083-351X
- ORCIDのPut Code : 45245352
- PubMed ID : 22665493
- SCOPUS ID : 84864392477
- Web of Science ID : WOS:000306916300058